NEB Tools Keyboard

NEB Interactive Tools

NEB Interactive Tools

  • Do you need advice choosing the right reaction buffer for your double digests?
  • Or do you plan a complex DNA assembly and you need a tool for your primer design?

We have numerous interactive online tools for these and other questions in your daily lab work.

NEB Tools also available as mobile app!


NEBcutter V2.0

Use this tool to identify the restriction sites within your DNA sequence. Choose between Type II and commercially available Type III restriction enzymes to digest your DNA. NEBcutter® V2.0 will indicate cut frequency and methylation state sensitivity.


NEBNext Selector

NEBNext Selector is a guide for selecting appropriate products for NextGen sequencing workflows.


Double Digest Finder

Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.



Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity.


Enzyme Finder

Use this tool to select restriction enzymes by name, sequence, overhang or type. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job.


Competitor Cross-Reference Tools

Use this tool to select another company’s product and find out which NEB product is compatible. Choose either the competitor’s product name or catalog number from the available selections, and this tool will identify the recommended NEB product.



Use this tool as a guide to the ever-changing landscape of restriction enzymes. REBASE, the Restriction Enzyme DataBASE, is a dynamic, curated database of restriction enzymes and related proteins.



Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.


Tm Calculator

Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.


NEBuilder Assembly Tool

NEBuilder® Assembly Tool can be used to design primers for your NEBuilder or Gibson Assembly reaction, based on the entered fragment sequences and the polymerase being used for amplification.


PCR Selection Tool

Use this tool to help select the right DNA polymerase for your PCR setup. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction.

Golden Gate Assembly

NEB Golden Gate Assembly Tool

Use this tool to assist with in silico DNA construct design for Golden Gate DNA assembly. It enables the accurate design of primers with appropriate type IIS restriction sites and overlaps, quick import of sequences in many formats and export of the final assembly, primers and settings.

PCR Fidelity Estimator

Estimate the percentage of correct DNA copies (those without base substitution errors) per cycle of PCR for selected DNA polymerases.



NEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the Q5® Site-Directed Mutagenesis Kit. This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches.

Icon Golden Gate Assembly Tool


Polbase is a repository of biochemical, genetic, and structural information about DNA Polymerases.


Thermostable Ligase Reaction Temperature Calculator

This tool will estimate an optimal reaction temperature to minimize mismatch for thermostable ligation of two adjacent ssDNA probes annealed to a template.


DNA Sequences & Maps Tools

Use this tool to find the nucleotide sequence files for commonly used molecular biology tools, including plasmid, viral and bacteriophage vectors.

Further information can be found in our Technical Resources section or at Information on trademarks can be found here.