NEB Interactive Tools
- Do you need advice choosing the right reaction buffer for your double digests?
- Or do you plan a complex DNA assembly and you need a tool for your primer design?
We have numerous interactive online tools for these and other questions in your daily lab work.
NEB Tools also available as mobile app!
Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.
This tool designs probes to be used with the NEBNext RNA Depletion Core Reagent Set or to supplement an existing NEBNext Depletion Kit for the depletion of unwanted RNA species.
Use this tool as a guide to the ever-changing landscape of restriction enzymes. REBASE, the Restriction Enzyme DataBASE, is a dynamic, curated database of restriction enzymes and related proteins.
Use this tool to select another company’s product and find out which NEB product is compatible. Choose either the competitor’s product name or catalog number from the available selections, and this tool will identify the recommended NEB product.
NEB LAMP Primer Design Tool can be used to design primers for your Loop-mediated Isothermal Amplification. Fixed primers can be specified for the design of LAMP primers, and subsequent Loop primers are then designed based on LAMP primer selection.
Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity.
Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.
Use this tool to help select the right DNA polymerase for your PCR setup. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction.
Use this tool to assist with in silico DNA construct design for Golden Gate DNA assembly. It enables the accurate design of primers with appropriate type IIS restriction sites and overlaps, quick import of sequences in many formats and export of the final assembly, primers and settings.
NEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the Q5® Site-Directed Mutagenesis Kit. This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches.
This tool will estimate an optimal reaction temperature to minimize mismatch for thermostable ligation of two adjacent ssDNA probes annealed to a template.
Use this tool to simplify the process of selecting the appropriate exonucleases for use in your nucleic acid digestion workflows. The tool guides you to product recommendations based on your answers to a few simple questions.