sgRNA Synthesis

Along with Cas9 nuclease, CRISPR experiments require the introduction of an sgRNA containing an approximately 20-base sequence specific to the target DNA 5′ of a non-variable scaffold sequence. sgRNA can be delivered as RNA or by transforming with a plasmid with the sgRNA-coding sequence under a promoter. A number of strategies have been developed to quickly swap out the 20 base sequences allowing convenient sgRNA cloning using NEB products.

Plasmids containing sgRNA sequences can be constructed using a variety of methods. Common to each is the requirement to introduce an approximately 20 base target sequence downstream of a promoter. Guide RNA templates can then be used as templates for in vitro transcription or directly introduced.

Product table

PRODUCT Prod. # SIZE PRICE*
EnGen® sgRNA Synthesis Kit S. pyogenes E3322S 20 rxns 302 €* In den Shop wechseln Produktinformationen
E3322V 10 rxns 155 €* In den Shop wechseln Produktinformationen
HiScribe™ T7 High Yield RNA Synthesis Kit E2040S 50 rxns 232 € In den Shop wechseln Produktinformationen
HiScribe™ T7 Quick High Yield RNA Synthesis Kit E2050S 50 rxns 284 € In den Shop wechseln Produktinformationen
Q5® High-Fidelity DNA Polymerase M0491S 100 units 101 € In den Shop wechseln Produktinformationen
M0491L 500 units 444 € In den Shop wechseln Produktinformationen
Q5® Hot Start High-Fidelity DNA Polymerase M0493S 100 units 118 € In den Shop wechseln Produktinformationen
M0493L 500 units 534 € In den Shop wechseln Produktinformationen

As of: 03.01.2022

* Permanent low prices – no further discounts apply. Offer closes 31.12.2022.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.