Genome Editing CRISPR Cas9

sgRNA Synthesis

Along with Cas9 nuclease, CRISPR experiments require the introduction of an sgRNA containing an approximately 20-base sequence specific to the target DNA 5′ of a non-variable scaffold sequence. sgRNA can be delivered as RNA or by transforming with a plasmid with the sgRNA-coding sequence under a promoter. A number of strategies have been developed to quickly swap out the 20 base sequences allowing convenient sgRNA cloning using NEB products.

EnGen sgRNA Synthesis Kit Overview

Plasmids containing sgRNA sequences can be constructed using a variety of methods. Common to each is the requirement to introduce an approximately 20 base target sequence downstream of a promoter. Guide RNA templates can then be used as templates for in vitro transcription or directly introduced.

sgRNA Synthesis In vitro Transcription

Product table

PRODUCT Prod. # SIZE PRICE*
EnGen® sgRNA Synthesis Kit S. pyogenes E3322S 20 rxns 299 €* In den Shop wechseln Produktinformationen
E3322V 10 rxns 149 €* In den Shop wechseln Produktinformationen
HiScribe™ T7 High Yield RNA Synthesis Kit E2040S 50 rxns 232 € In den Shop wechseln Produktinformationen
HiScribe™ T7 Quick High Yield RNA Synthesis Kit E2050S 50 rxns 284 € In den Shop wechseln Produktinformationen
Q5® High-Fidelity DNA Polymerase M0491S 100 units 99 € In den Shop wechseln Produktinformationen
M0491L 500 units 435 € In den Shop wechseln Produktinformationen
Q5® Hot Start High-Fidelity DNA Polymerase M0493S 100 units 115 € In den Shop wechseln Produktinformationen
M0493L 500 units 521 € In den Shop wechseln Produktinformationen

As of: 01.01.2021

* Permanent low prices – no further discounts apply. Offer closes 31.12.2021.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.