PURExpress In Vitro Protein Synthesis Kit

A rapid method for gene expression analysis.

A rapid method for gene expression analysis, PURExpress is a novel cell-free transcription/ translation system reconstituted from the purified components necessary for E. coli translation. The nuclease-free and protease-free nature of the PURExpress platform preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.

PURExpress is based on the PURE system technology originally developed by Dr. Takuya Ueda at the University of Tokyo and commercialized as the PURESYSTEM® by Biocomber
(Tokyo, Japan).

E6800c
Advantages:
  • Cleaner system - lack of endogenous proteases or nucleases eliminates sample degradation
  • Flexible - formulation amenable to modification
  • Simple analysis - synthesized protein can often be visualized on a Coomassie stained gel
  • Easy-to-use - requires the mixing of two tubes followed by the addition of template DNA, either circular or linear template
  • Fast - reaction is complete in approximately two hours
  • Efficient - purified components enable more control of translational and folding machinery
  • Stable - resistant to multiple freeze/ thaw cycles

Expression and reverse purification of DHFR (A) and T4 DNA Ligase (B) using PURExpress.
125 μl reactions were carried out according to recommendations in the accompanying manual. Samples were analyzed on a 10–20% Tris-glycine gel and stained with Coomassie Blue. Note that in both cases, the desired protein can be visualized in the total protein fraction. The red dot indicates the protein of interest. Marker M: Protein Ladder #P7703

Product table

PRODUCT Prod. # SIZE INFO
PURExpress® In vitro Protein Synthesis Kit E6800S 10 rxns In den Shop wechseln Produktinformationen
E6800L 100 rxns In den Shop wechseln Produktinformationen
PURExpress® Delta Ribosome Kit E3313S 10 rxns In den Shop wechseln Produktinformationen
PURExpress® Delta (aa, tRNA) Kit E6840S 10 rxns In den Shop wechseln Produktinformationen
PURExpress® Delta RF123 Kit E6850S 10 rxns In den Shop wechseln Produktinformationen
Passend dazu:
PURExpress® Disulfide Bond Enhancer E6820S 50 rxns In den Shop wechseln Produktinformationen
E. coli Ribosome P0763S 1 mg In den Shop wechseln Produktinformationen
Murine RNase Inhibitor M0314S 3.000 units In den Shop wechseln Produktinformationen
M0314L 15.000 units In den Shop wechseln Produktinformationen

As of: 23.01.2024

Licenses: PURExpress® Disulfide Bond Enhancer (E6820) is licensed from BioComber (Tokyo, Japan) under Patent No. WO2005-105994. Commercial use of PURExpress® Disulfide Bond Enhancer requires a license from New England Biolabs, Inc. This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

PURExpress Kit Components

IN VITRO PROTEIN SYNTHESIS
(E6800S)		 ▲ RIBOSOME
(E3313S)		 ▲ RF 123
(E6850S)		 ▲ (aa, tRNA)
(E6840S)
Solution A Solution A Solution A Solution A
(minus aa and tRNA)
Solution B Factor Mix Solution B
(minus RF1, RF2 and RF3)		 Solution B
Control (DHFR) template Control (DHFR) template Control (DHFR) template Control (DHFR) template
Control Ribosomes RF1, RF2 and RF3 Amino Acid Mixture
E. coli tRNA

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.