Quickly and easily purify high quality DNA from PCR and other enzymatic reactions
The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times.
Protocol Video Monarch PCR & DNA Cleanup
Designed with sustainability in mind
We achieve this by using particularly thin-walled columns and bottles. In this way we reduce the use of plastic and thus the amount of waste in the laboratory. In addition, all components of the packaging are made of recycled material.
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Monarch columns are designed for performance
NEB’s Monarch Nucleic Acid Purification Kits are optimized for maximum performance and minimal environmental impact. Our unique thin-walled column design uses less plastic, prevents buffer retention, eliminates the risk of carryover contamination, and enables elution in smaller volumes.
Suitable for cleanup of oligos and short DNA fragments
The Monarch PCR & DNA Cleanup Kit protocol can be modified to enable the purification of ssDNA, oligonucleotides, and other small DNA fragments. The Oligonucleotide Cleanup protocol efficiently removes unincorporated nucleotides, short oligos, dyes, enzymes, and salts from labeling and other enzymatic reactions. The modified protocol utilizes the same columns and bind/wash/elute workflow of the Monarch PCR & DNA Cleanup Kit with > 70% recovery and cleanup of oligonucleotides ≥ 15 bp (dsDNA) or ≥18 nt (ssDNA).
Synthesized ssDNA and dsDNA oligonucleotides (1 µg in 50 µl H2O) of varying lengths (6-20 nt) were purified using the Oligonucleotide Cleanup Protocol and the Monarch PCR & DNA Cleanup Kit (NEB #T1030) and were eluted in 50 µl water. The average percent recovery (n=3) of the oligonucleotides was calculated from the resulting A260 as measured using a Trinean DropSense™ 16. Use of the Oligonucleotide Cleanup Protocol and the Monarch PCR & DNA Cleanup Kit (NEB #T1030) results in the efficient removal of small oligonucleotides (6-12 nt) and > 70% recovery and cleanup of oligonucleotides ≥ 15 bp (dsDNA) or ≥ 18 nt (ssDNA).
Tips for using the Monarch PCR & DNA Cleanup Kit
Optimize your DNA isolation from PCR and other enzymatic reactions with our quick tips for using the Monarch PCR & DNA Cleanup Kit.
Video: PCR & DNA Cleanup Tips
Available Monarch DNA Cleanup products:
| PRODUCT | Prod. # | SIZE | INFO | |
|---|---|---|---|---|
| Monarch PCR & DNA Cleanup Kit (5 μg)* | T1030S/L | 50 / 250 preps |  |
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| Monarch DNA Cleanup Binding Buffer | T1031L | 235 ml | |
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| Monarch DNA Elution Buffer | T1016L | 25 ml | |
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| Monarch DNA Wash Buffer | T1032L | 25 ml | |
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| Monarch DNA Cleanup Columns | T1034L | 100 preps | |
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As of: 01.01.2024
*Permanent low prices – no further discounts apply. Offer closes 31.12.2024
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
