The Monarch RNA Cleanup Kits provide a fast and simple silica spin column-based solution for RNA cleanup and concentration after any enzymatic reaction (including in vitro transcription, DNase I treatment, capping and labeling) and after other purification methods such as phenol/chloroform extraction.
The Monarch RNA Cleanup Kits are available in 3 different binding capacities:
Each kit contains unique columns, all designed to prevent buffer retention and ensure no carryover of contaminants, enabling low-volume elution of highly-pure RNA. Following the standard protocol, RNA ≥ 25 nt is purified with this kit; however, a modified protocol is available to enable the binding of RNA as small as 15 nt (including miRNAs).
Reasons to choose Monarch for RNA Cleanup:
- Choose from 3 different binding capacities and elution volumes
- Quickly and easily purify large quantities of high-quality RNA from in vitro transcription (IVT) reactions
- Efficiently remove unincorporated nucleotides from your RNA sample
|RNA Cleanup and Concentration (including from the TRIzol aqueous phase)||RNA purified by other methods can be further purified|
|Enzymatic Reaction Cleanup||Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting|
|In vitro Transcription Cleanup||Enzymes and excess NTPs are removed to yield highly pure synthesized RNA|
|RNA Gel Extraction||Purification of RNA from agarose gels|
|RNA Fractionation||Fractionation of RNA into small and large RNA pools|