The Monarch Mag PCR & DNA Cleanup Kit (5 µg) offers rapid, reliable DNA purification with a flexible magnetic bead-based workflow. Efficiently scale your DNA cleanup processes for high-throughput applications while ensuring consistent, high-quality results across a wide range of sample types.
- Magnetic bead-based kit for PCR and DNA cleanup and purification
- Purify up to 5 μg of DNA
- Compatible with manual and automated systems
- No pH monitoring needed
- Optimized protocols for purifying oligonucleotides, genomic DNA, and RCA products
Monarch Mag PCR & DNA Cleanup Kit (5 µg) enables versatile purification of diverse DNA types
Monarch Mag PCR & DNA Cleanup Kit (5 µg) (NEB #T4130) enables versatile purification of diverse DNA types. DNA samples were purified in triplicate using the Monarch Mag PCR & DNA Cleanup Kit (5 µg) with a manual workflow, following either the standard protocol (for linear, circular and single-stranded DNA) or the supplemental protocol (for large DNA fragments, oligonucleotides and RCA products). Each sample contained 1 µg of DNA and was eluted in 30 µl of Monarch Buffer EY, except for the RCA product (20 µl at 1 mg/ml). DNA concentrations for both input and eluted samples were measured using the Trinean DropSense 16, except for the RCA product, which was quantified using the Qubit® dsDNA Broad Range Assay Kit. Percent recovery was calculated based on measured DNA concentrations and elution volumes, and the results are presented in a bar graph.
Product table:
| PRODUCT | Art.Nr.: | SIZE | INFO | |
|---|---|---|---|---|
| Monarch Mag PCR & DNA Cleanup Kit (5 μg) | T4130S | 50 preps |  |
 |
| Monarch Mag PCR & DNA Cleanup Kit (5 μg) | T4130L | 250 preps | |
|
| Monarch Mag PCR & DNA Cleanup Kit (5 μg) | T4130V | 100 preps | |
|
| Monarch Mag Beads M2 | T4105S | 9 ml | |
|
| Monarch Buffer BZ | T1114L | 168 ml | |
|
| Monarch Buffer WZ | T1115L | 26 ml | |
|
| Monarch Buffer EY** | T1116L | * | ||
*Product not sold separately.
**The EY buffer consists of 10 mM Tris and 0.1 mM EDTA, adjusted to pH 8.5. Alternatively, you may use a comparable low-salt buffer for elution or simply nuclease-free water (#B1500S/L).
As of: 25.11.2025
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
