The Monarch Mag Cell-free DNA (cfDNA) Extraction Kit is a magnetic bead-based kit for a reproducible extraction of circulating cell-free DNA from biofluids. The silica-coated magnetic bead method ensures optimal cfDNA binding and recovery for sensitive downstream detection.
When integrated with NEB’s sequencing and amplification applications, our cfDNA extraction solution enables comprehensive and streamlined workflows to generate high-quality data from challenging biofluid samples.
- Efficient isolation of circulating cell-free DNA from plasma, urine, and cerebrospinal fluid
- Low elution volumes (≥15 μl) for high DNA concentration
- Efficient recovery of cfDNA in the typical size range (50–300 bp)
- Adaptable sample input volume (1–4 ml)
- Magnetic bead-based kit for easy upscaling and automation (including KingFisher® Flex and other platforms)
Monarch Mag Cell-free DNA (cfDNA) Extraction Kit enables higher yield compared to other leading suppliers
Plasma was spiked with Low Molecular Weight ladder (NEB #N3233S), to simulate fragmented cfDNA, and was subsequently extracted using the Monarch Mag Cell-free DNA (cfDNA) Extraction Kit and two commercially available cfDNA extraction kits. The Monarch Kit recovers a higher yield in the typical cfDNA fragment region (150–300 bp), as well as of shorter DNA fragments (<150 bp).
| PRODUCT | Art.Nr.: | SIZE | PRICE | ||
|---|---|---|---|---|---|
| Monarch Mag Cell-free DNA (cfDNA) Extraction Kit | T4070V | 20 preps | 235 € |  |
 |
| Monarch Mag Cell-free DNA (cfDNA) Extraction Kit | T4070S | 100 preps | 1060 € | |
|
| Monarch StabiLyse DNA/RNA Buffer | T2111 | – | – | |
|
| Monarch Buffer BX | T2041 | – | – | |
|
| Monarch Buffer BY | T1121 | – | – | |
|
| Monarch Buffer WZ | T1115 | – | – | |
|
| Nuclease-free Water | B1500 | – | – | |
|
| Monarch Mag Beads M2 | T4105 | – | – | |
|
| Proteinase K, Molecular Biology Grade | P8200SVIAL | – | – | |
|
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
