Ligase Header

Ligase Master Mixes

Blunt/TA Ligase Master Mix

The Blunt/TA Ligase Master Mix (NEB #M0367) is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. This master mix format simplifies reaction setup, ensuring robust, rapid ligation with an optimized ratio of enzyme and buffer components. Reactions can be used to directly transform many strains of chemically competent E. coli without dilution.

Blunt/TA Ligase Master Mix Outperforms the Competition:

duplicate_ligation_reactions2

Duplicate ligation reactions of blunt or T/A vector/insert pairs were set upaccording to the master mix vendors’ suggestions. Equal amounts of ligated DNA were used to transform NEB 10-beta Competent E. coli (NEB #C3019) and triplicate plating was performed. Transformation results were averaged and graphed as a percentage of the highest performing reaction, T/A ligation using the Blunt/TA Ligase Master Mix.

Instant Sticky-end Ligase Master Mix

Complete with T4 DNA Ligase (NEB #M0202), proprietary ligation enhancer and optimized reaction buffer, the Instant Sticky-end Ligase Master Mix (NEB #M0370) enables instant, incubation- free ligation. Specifically formulated to rapidly ligate sticky-end (2–4 bp) substrates and improve transformation, this mix simplifies reaction set-up and reduces the time needed for routine ligations. Reactions can be used to directly transform many strains of chemically competent E. coli without dilution.

Instant Sticky-end Ligase Master Mix Offers Robust Ligation with No Incubation Step

Reactions containing 20 ng of pUC19 digested with SacI/SphI, and a 3-foldmolar excess of a 1.6 kb fragment from a different plasmid with compatible ends were set up using the Instant Sticky-end Ligase Master Mix. Without any additional incubation time, 2 µl were immediately withdrawn and used to transform a 50 µl aliquot of NEB 10-beta Competent E. coli (NEB #C3019). A 50 µl aliquot of the 1 ml outgrowth was plated onto a selective plate and incubated overnight at 37°C. Results show that several hundred colonies wereproduced.

P R O D U C T T A B L E

Product table

PRODUCT Prod. # SIZE PRICE
Blunt/TA Ligase Master Mix M0367S 50 rxn 100 € In den Shop wechseln Produktinformationen
M0367L 250 rxn 400 € In den Shop wechseln Produktinformationen
Instant Sticky-end Ligase Master Mix M0370S 50 rxn 100 € In den Shop wechseln Produktinformationen
M0370L 250 rxn 400 € In den Shop wechseln Produktinformationen
ElectroLigase™ M0369S 50 rxn 121 € In den Shop wechseln Produktinformationen
T4 DNA Ligase M0202S 20.000 units 68 € In den Shop wechseln Produktinformationen
M0202L 100.000 units 272 € In den Shop wechseln Produktinformationen
T4 DNA Ligase, conc. M0202T 20.000 units 68 € In den Shop wechseln Produktinformationen
M0202M 100.000 units 272 € In den Shop wechseln Produktinformationen
Quick Ligation™ Kit M2200S 30 reactions 99 € In den Shop wechseln Produktinformationen
M2200L 150 reactions 396 € In den Shop wechseln Produktinformationen
T3 DNA Ligase M0317S 100.000 units 68 € In den Shop wechseln Produktinformationen
M0317L 750.000 units 272 € In den Shop wechseln Produktinformationen
T7 DNA Ligase M0318S 100.000 units 68 € In den Shop wechseln Produktinformationen
M0318L 750.000 units 272 € In den Shop wechseln Produktinformationen
E. coli DNA Ligase M0205S 200 units 62 € In den Shop wechseln Produktinformationen
M0205L 1.000 units 248 € In den Shop wechseln Produktinformationen
Taq DNA Ligase M0208S 2.000 units 80 € In den Shop wechseln Produktinformationen
M0208L 10.000 units 320 € In den Shop wechseln Produktinformationen
9°N™ DNA Ligase M0238S 2.500 units 80 € In den Shop wechseln Produktinformationen

The Ligation Module for Library Preparation in Next-Gen-Sequencing

PRODUCT Prod. # SIZE PRICE
NEBNext® Quick Ligation Module E6056S 20 rxns 306 € In den Shop wechseln Produktinformationen
E6056L 100 rxns 1.224 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.