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DNA Ligases

Ligation of DNA is a critical step in many modern molecular biology workflows. The sealing of nicks between adjacent residues of a single-strand break on a double-strand substrate and the joining of double-strand breaks are enzymatically catalyzed by DNA ligases.

For the full list of DNA Ligases and Ligase Master Mixes offered by NEB, as well as suggested applications, please use our DNA Ligase Selection Chart.

Ligase Selection Chart

Advantages

  • Fast ligations
  • Robust ligation efficiency
  • Industry standard for purity
  • Most extensive selection commercially available
  • New formulations optimized for your substrates
  • Convenient master mix formats
T4 DNA Ligase Gelbild

Extreme purity with NEB’s T4 DNA Ligase
Equivalent amounts of protein were loaded and silver stained using SilverXpress™. Marker M is NEB’s Broad Range Protein Marker (NEB #P7702).

 T4 DNA Ligase Quality Control
Physical Purity Determined by SDS-PAGE analysis (silver stained gel) (Figure 1).
Exonuclease Activity Contaminating exonucleases are assayed by monitoring release of radioactively labeled DNA substrate. Very low levels of specific types of exonuclease contamination are also assayed for using capillary electrophoresis with dye labeled substrate (Figure 2).
Nuclease Activity Contaminating nucleases are assayed by incubation with HindIII fragments of lambda DNA.
Endonuclease Activity Incubation with supercoiled plasmid DNA for 4 hours is used to detect any nicking or nonspecific nuclease degradation.
Functional Assay Room temperature ligation is performed.

Product table

PRODUCT Prod. # SIZE PRICE
T4 DNA Ligase M0202S 20.000 units 68 € In den Shop wechseln Produktinformationen
M0202L 100.000 units 272 € In den Shop wechseln Produktinformationen
T4 DNA Ligase, conc. M0202T 20.000 units 68 € In den Shop wechseln Produktinformationen
M0202M 100.000 units 272 € In den Shop wechseln Produktinformationen
Quick Ligation™ Kit M2200S 30 reactions 99 € In den Shop wechseln Produktinformationen
M2200L 150 reactions 396 € In den Shop wechseln Produktinformationen
T7 DNA Ligase M0318S 100.000 units 70 € In den Shop wechseln Produktinformationen
M0318L 750,000 units 281 € In den Shop wechseln Produktinformationen
T3 DNA Ligase M0317S 100.000 units 70 € In den Shop wechseln Produktinformationen
M0317L 750.000 units 281 € In den Shop wechseln Produktinformationen
Taq DNA Ligase M0208S 2.000 units 82 € In den Shop wechseln Produktinformationen
M0208L 10.000 units 328 € In den Shop wechseln Produktinformationen
ElectroLigase M0369S 50 reactions 121 € In den Shop wechseln Produktinformationen
HiFi Taq DNA Ligase M0647S 50 reactions 160 € In den Shop wechseln Produktinformationen
Hi-T4 DNA Ligase M2622S 20.000 units 68 € In den Shop wechseln Produktinformationen
M2622L 100.000 units 272 € In den Shop wechseln Produktinformationen
Salt-T4 DNA Ligase M0467S 20.000 units 68 € In den Shop wechseln Produktinformationen
M0467L 100.000 units 272 € In den Shop wechseln Produktinformationen
SplintR Ligase M0375S 1.250 units 98 € In den Shop wechseln Produktinformationen
M0375L 6.250 units 439 € In den Shop wechseln Produktinformationen

As of: 01.01.2021

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.