FastCloning with reagents from NEB

Accelerate your cloning experiments with reagents from NEB

While cloning has typically been a time-consuming process in the past, advances in both technologies and reagents have enabled the process to take much less time. Race to NEB for the highest quality products that save valuable time!

Fast cloning reagents from NEB:

SEAMLESS CLONING

NEBuilder HiFi DNA Assembly Master Mix, NEBuilder HiFi DNA Assembly Cloning Kit & NEBuilder HiFi DNA Assembly Bundle for Large Fragments

Rapid, seamless assembly/cloning of multiple fragments in as little as 15 minute

NEB® Golden Gate Assembly Mix (BsaI-HF®v2)

Efficient, ordered assembly of multiple fragments in a single reaction

DIGESTION

High-Fidelity (HF) restriction enzymes

HF enzymes offer reduced star activity, rapid digestion and 100% activity in CutSmart® Buffer

Time-Saver restriction enzymes

>195 enzymes are Time-Saver qualified and will digest DNA in 5-15 minutes; digest overnight with no unwanted star activity

END MODIFICATION

Quick CIP

Complete dephosphorylation in 10 minutes with rapid, irreversible heat inactivation

Quick Blunting Kit

Blunt restriction enzyme-digested DNA in less than 30 minutes at room temperature

AMPLIFICATION

Q5 High-Fidelity DNA Polymerases

With the lowest error rate of all known commercially available PCR polymerases, Q5 enables extension times as low as 10 seconds per kb on templates up to 20 kb

PURIFICATION

Monarch DNA Purification Kits

Purify high-quality DNA in minutes (5 minutes for the PCR & DNA Cleanup Kit!). Designed with sustainability in mind, Monarch kits use less plastic and responsibly-sourced packaging

LIGATION

Instant Sticky-end Ligase Master Mix

Specially formulated to instantly ligate 2-4 basepair overhangs! Just add DNA, flick the tube, and ligation occurs instantly! No need for incubation.

Blunt/TA Ligase Master Mix

Specially formulated to ligate single-base and blunt ends in just 15 minutes

Quick Ligation Kit

Ligates both cohesive and blunt ends in just 5 minutes at room temperature

TRANSFORMATION

NEB Turbo Competent E. coli (High-Efficiency)

Generate colonies in as little as 6.5 hours with a 5-minute transformation protocol

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.