The Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) is a high-capacity version of the Monarch Spin PCR & DNA Cleanup Kit (5 μg) offering class-leading binding capacity. It efficiently purifies and concentrates up to 100 μg of high-quality, double-stranded and single-stranded DNA.
Request your free sample:
- Purify up to 100 μg of DNA
- Elute in just 50 μl for highly concentrated DNA
- No pH monitoring required
- Modified protocol provided for oligonucleotide, genomic DNA, or RCA products, enabling efficient purification of various DNA samples
Designed with sustainability in mind
The kit offers significantly less plastic through uniquely designed columns and bottles with plastic reduction while still maintaining structural integrity for centrifugation or vacuum manifolds and other steps.
Kit boxes, inserts, and protocol cards are made of recycled paper.
Unique Column Design
NEB Monarch’s unique column design and membrane assembly allows high-quality, highly-concentrated nucleic acid purification with low elution volume for downstream applications. The column is designed and made with significantly less plastic for a reduced environmental impact.
Monarch Spin High-Capacity DNA Cleanup Kit (100 µg) effectively recovers various DNA sample types, maximizing utility of the kit
DNA samples were purified in triplicate using the Monarch Spin High-Capacity DNA Cleanup Kit (100 µg), following either the standard protocol (for linear, circular, and ssDNA) or the supplemental protocol (for large DNA). Each sample, except for the RCA product (50 µl, 1 mg/ml), contained 10 µg of DNA and was eluted in 100 µl of Monarch Buffer EY. DNA concentrations for both input and eluted samples were measured using a Trinean DropSense 16, except for the RCA product, which was measured using the Qubit dsDNA Broad Range (BR) Assay Kit.
Monarch Spin High-Capacity DNA Cleanup Products:
| PRODUCT | Prod. # | SIZE | INFO | |
|---|---|---|---|---|
| Monarch Spin High-Capacity DNA Cleanup Kit (100 µg) | T1135L | 200 preps |  |
 |
| T1135S | 50 preps | |||
| T1135V | 10 preps | |||
| Monarch Spin Columns S3A and Tubes | T1157L | 100 preps | |
|
| Monarch Spin Collection Tubes | T2118L | 100 preps | |
|
| Monarch Buffer BZ | T1114L | 168 ml | |
|
| Monarch Buffer WZ | T1115L | 26 ml | |
|
As of: 31.07.2025
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
