Successfully see more small RNAs
Bias in small RNA library prep can seriously impact the accuracy of your data, skewing the representation of rare RNA species. The NEBNext Low-bias Small RNA Library Prep Kit minimizes biased representation of small RNAs, more accurately reflecting the number and proportion of unique small RNAs present. NEB’s next-generation kit utilizes a streamlined workflow for the capture of small RNAs (<120 nt) with a 5´ phosphate and 3´ hydroxyl group, and can be used to identify microRNAs (miRNAs), transfer RNAs (tRNAs) and tRNA-derived fragments, small nucleolar RNAs (snoRNAs), piwi-interacting RNAs (piRNAs), and plant miRNAs. Designed for the preparation of Illumina-compatible small RNA sequencing libraries, this latest NEBNext kit is kind of a big deal!
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- Analyze all RNA species present without library prep-generated bias
- Simplify and streamline your library prep workflow (~3.5 hours), saving time
- Derive insights from biologically relevant input amounts
- Learn a single protocol for standard and 2´-O-methylated samples
- Expand your insights with up to 480 compatible UDI primer pairs, available separately
NEBNext Low-bias Small RNA Library Prep workflow:
NEBNext Low-bias Small RNA Library Prep Kit produces libraries with the lowest bias:
NEBNext Low-bias Small RNA libraries were made using a mix of 100 synthetic control miRNAs, including five that had 3´ 2´-O-methyl ends. Libraries were prepared from 0.3 ng of the synthetic miRNA mix to compare library-generated bias between the NEBNext Low-bias Small RNA Library Prep Kit and small RNA kits from Revvity (NEXTFLEX Small RNA Sequencing Kit V4), Qiagen (QIAseq miRNA Library Kit), and Illumina (TruSeq Small RNA Library Prep). Libraries were sequenced on an Illumina NextSeq 500 (1 x 56 bases), and expected reads were calculated from total reads mapped to the synthetic controls, divided by the total number of control sequences (black line at 100%). Percent of observed reads to expected reads was calculated for each control sequence and plotted across replicates.
Products
| PRODUCT | Prod. # | SIZE | INFO | |
|---|---|---|---|---|
| NEBNext Low-bias Small RNA Library Prep Kit | E3420S | 24 rxns |  |
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| E3420L | 96 rxns | |
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As of: 28.07.2025
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
