Multiplexing Strategy with NEBNext Oligos/Adaptors

Find the best Oligo Strategy for your experiment!

NEBNext Multiplex Oligos Selection Chart

 

Single Index In den Shop wechseln Dual Index In den Shop wechseln Unique Dual Index In den Shop wechseln Unique Dual Index UMIs
In den Shop wechseln
NEB Products NEB #E7335,
NEB #E7500,
NEB #E7710,
NEB #E7730,
NEB #E6609
NEB #E7600,
NEB #E7780
NEB #E6440,
NEB #E6442,
NEB #E6444,
NEB #E6446,
NEB #E7140
NEB #E7395,
NEB #E7416
Contains UMI No No No Yes
Addresses index hopping No No Yes Yes
Indexing Strategy Index Primer Index Primer Index Primer Index Adaptor
Applications DNA-seq, RNA-seq
(except Small RNA)
DNA-seq, RNA-seq
(except Small RNA)
DNA-seq, RNA-seq
(except Small RNA)
PCR-free DNA-seq, RNA-seq
(except Small RNA)
Number of indices for multiplexing up to 96 up to 384 up to 384 up to 96
Compatible with EM-seq Yes* Yes* Yes* No
Compatible with EpiMark bisulfite sequencing Yes** Yes** Yes** No**
Number of sets available; formats and indices available Five; Sets 1-4 (12 indices/set): Individual vials 96 Index: Premixed plate Two; individual vials containing 8 i5 primers and 12 i7 primers for combinatorial mixing Four; 96 index combinations, premixed in foil-sealed 96-well plates; Including a version for EM-seq (up to 120 indices, either 96-well plate or 24 vial format) One; 96 index adaptor combinations, premixed in foil-sealed 96 well plate (DNA-seq OR RNA-seq) and primers

* Requires the use of the EM-seq Adaptor; Single, dual and unique dual index are all compatible; NEB recommends using the Unique Dual Index Primers found in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) or the NEBNext Multiplex Oligos for EM-seq (NEB #E7140), both supplied with the NEBNext EM-seq Adaptor; For higher levels of multiplexing, Unique Dual Index Primers Sets 3 and 4  (NEB #E6444 and #E6446) are also validated for EM-seq.

**  Requires use of NEBNext EM-seq adaptor from NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs, #E7140S/L or NEBNext methylated adaptor from NEBNext Multiplex Oligos for Illumina (Methylated Adaptor, Index Primers Set 1, #E7535S/L) .

Available NEBNext (Mulitplex-) Oligos for Illumina

PRDOUCT Art. # SIZE PRICE
NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) E7335S 24 rxns 106 € In den Shop wechseln Produktinformationen
E7335L 96 rxns 384 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (Index Primer Set 2) E7500S 24 rxns 106 € In den Shop wechseln Produktinformationen
E7500L 96 rxns 384 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (Index Primer Set 3) E7710S 24 rxns 106 € In den Shop wechseln Produktinformationen
E7710L 96 rxns 384 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (Index Primer Set 4) E7730S 24 rxns 106 € In den Shop wechseln Produktinformationen
E7730L 96 rxns 384 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (96 Index Primers) E6609S 96 rxns 680 € In den Shop wechseln Produktinformationen
E6609L 384 rxns 2.720 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (Dual Index Set 1) E7600S 96 rxns 461 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (Dual Index Set 2) E7780S 96 rxns 461 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 1) E6440S 96 rxns 535 € In den Shop wechseln Produktinformationen
E6440L 384 rxns 1.925 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2) E6442S 96 rxns 535 € In den Shop wechseln Produktinformationen
E6442L 384 rxns 1.925 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 3) E6444S 96 rxns 535 € In den Shop wechseln Produktinformationen
E6444L 384 rxns 1.925 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 4) E6446S 96 rxns 535 € In den Shop wechseln Produktinformationen
E6446L 384 rxns 1.925 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) E7140S 24 rxns 135 € In den Shop wechseln Produktinformationen
E7140L 96 rxns 535 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) E7395S 96 rxns 672 € In den Shop wechseln Produktinformationen
E7395L 384 rxns 2.553 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1) E7416S 96 rxns 648 € In den Shop wechseln Produktinformationen
E7416L 384 rxns 2.460 € In den Shop wechseln Produktinformationen

As of: 01.01.2021

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.