NEBNext RNA Depletion

NEBNext rRNA & Globin mRNA Depletion Kits

NEBNext rRNA Depletion Kit

The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) enables efficient removal of ribosomal RNA (rRNA) from total RNA for human, mouse and rat RNA samples.

This kit works well for both low-quality, degraded RNA (including FFPE RNA) and high-quality, intact RNA. rRNA depletion with a broad range of input amounts (10 ng to 1 μg) is completed in approximately 2 hours, with less than 10 minutes of hands-on time.

Advantages:
  • Suitable for FFPE (degraded) or high-quality (intact) RNA
  • 10 ng to 1 μg input
  • Remove > 95% of rRNA, and obtain more relevant sequence reads from your sample
  • Obtain a more complete transcriptome picture through retention of noncoding & incomplete RNAs that are lost with oligo d(T) poly(A) mRNA enrichment methods
  • Suitable for use with human, mouse or rat samples
  • Easily integrated upstream of any downstream random-primed cDNA synthesis protocol
  • Also available with RNA Sample Purification Beads

Application Note:
Selective depletion of abundant RNAs to enable transcriptome analysis of low-input and highly-degraded RNA from FFPE breast cancer samples

cover_appnote_rrnadepletionkit
PDF Download

NEBNext rRNA Depletion Kit Workflow
Click to enlarge

NEB_NEBNext rRNA Depletion Workflow

New: NEBNext RNA Depletion Core Reagent Set

In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool.

The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts.

The kit is also available with RNAClean® beads.

Advantages:
  • Compatible with a broad range of input amounts: 10 ng - 1µg
  • Suitable for low-quality or high-quality RNA
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

New: NEBNext Globin & rRNA Depletion Kit

Globin mRNA and rRNA are highly abundant in blood samples, and their efficient and specific removal is desirable in order to reveal the biological significance of less abundant transcripts.

The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete globin mRNA, cytoplasmic rRNA and mitochondrial rRNA with human, mouse and rat samples, and is effective with intact and degraded RNA.

Advantages:
  • Efficient, specific depletion of globin mRNA and rRNA
  • Suitable for low-quality or high-quality RNA
  • Compatible with a broad range of input amounts: 10 ng - 1 μg
  • Optional integration with poly(A) mRNA isolation workflows for removal of globin RNAs, rRNAs, and noncoding RNAs
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time
  • Also available with RNA Sample Purification Beads

New: NEBNext rRNA Depletion Kit (Bacteria)

Ribosomal RNA (rRNA) is highly abundant in bacterial samples, and its removal is desirable in order to reveal the biological significance of less abundant transcripts. Specific enrichment of bacterial mRNAs is challenging due to their lack of poly(A) tails, and so the converse – efficient and specific removal of bacterial rRNA – is necessary.

The NEBNext rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic).

„The NEB Bacterial Depletion has depleted rRNA equally or better than our previous ribodepletion gold standard across a wide RIN quality range. We have been pleased with the flexibility of Total RNA input ranges and have routinely gotten effective ribodepletion at 100 ng Total RNA Input in both single isolates and metagenomic samples. The protocol is also more ergonomically friendly than bead based ribodepletion protocols. Of all the new bacterial ribodepletion methods we have tested, NEB was by far the best.“

Research Assistant, Biomedical research institution

Advantages:
  • Efficient, specific depletion of bacterial rRNA (5S, 16S, 23S)
  • Compatible with both gram-positive and gram-negative organisms
  • Effective with monocultures and mix of bacterial species (e.g., metatranscriptome)
  • Compatible with a broad range of input amounts: 10 ng - 1 µg
  • Suitable for low-quality or high-quality RNA
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

Available products:

PRODUCT Prod. # SIZE PRICE
NEBNext® RNA Depletion Core Reagent Set E7865S 6 rxns 254 € In den Shop wechseln Produktinformationen
E7865L 24 rxns 924 € In den Shop wechseln Produktinformationen
E7865X 96 rxns 3.326 € In den Shop wechseln Produktinformationen
NEBNext® RNA Depletion Core Reagent Set with Beads E7870S 6 rxns 262 € In den Shop wechseln Produktinformationen
E7870L 24 rxns 963 € In den Shop wechseln Produktinformationen
E7870X 96 rxns 3.465 € In den Shop wechseln Produktinformationen
NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) E7400S 6 rxns 297 € In den Shop wechseln Produktinformationen
E7400L 24 rxns 1.080 € In den Shop wechseln Produktinformationen
E7400X 96 rxns 3.885 € In den Shop wechseln Produktinformationen
NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with Beads E7405S 6 rxns 305 € In den Shop wechseln Produktinformationen
E7405L 24 rxns 1.125 € In den Shop wechseln Produktinformationen
E7405X 96 rxns 4.050 € In den Shop wechseln Produktinformationen
NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) E6310S 6 rxns 330 € In den Shop wechseln Produktinformationen
E6310L 24 rxns 1.200 € In den Shop wechseln Produktinformationen
E6310X 96 rxns 4.320 € In den Shop wechseln Produktinformationen
NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with Beads E6350S 6 rxns 360 € In den Shop wechseln Produktinformationen
E6350L 24 rxns 1.324 € In den Shop wechseln Produktinformationen
E6350X 96 rxns 4.766 € In den Shop wechseln Produktinformationen
NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) E7750S 6 rxns 340 € In den Shop wechseln Produktinformationen
E7750L 24 rxns 1.235 € In den Shop wechseln Produktinformationen
E7750X 96 rxns 4.450 € In den Shop wechseln Produktinformationen
NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads E7755S 6 rxns 350 € In den Shop wechseln Produktinformationen
E7755L 24 rxns 1.290 € In den Shop wechseln Produktinformationen
E7755X 96 rxns 4.635 € In den Shop wechseln Produktinformationen
NEBNext® rRNA Depletion Kit (Bacteria) E7850S 6 rxns 297 € In den Shop wechseln Produktinformationen
E7850L 24 rxns 1.080 € In den Shop wechseln Produktinformationen
E7850X 96 rxns 3.885 € In den Shop wechseln Produktinformationen
NEBNext® rRNA Depletion Kit (Bacteria) with RNA Sample Beads E7860S 6 rxns 305 € In den Shop wechseln Produktinformationen
E7860L 24 rxns 1.125 € In den Shop wechseln Produktinformationen
E7860X 96 rxns 4.050 € In den Shop wechseln Produktinformationen
You may also be interested in:
NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina E7760S 24 rxns 1.028 € In den Shop wechseln Produktinformationen
E7760L 96 rxns 3.495 € In den Shop wechseln Produktinformationen
NEBNext® Ultra II RNA Library Prep Kit for Illumina® E7770S 24 rxns 980 €
In den Shop wechseln Produktinformationen
E7770L 96 rxns 3.325 € In den Shop wechseln Produktinformationen
NEBNext® Poly(A) mRNA Magnetic Isolation Module E7490S 24 rxns 67 € In den Shop wechseln Produktinformationen
E7490L 96 rxns 242 € In den Shop wechseln Produktinformationen

As of: 14.10.2020
NEBNext products are available at a permanent low price that can not be further discounted.

Request a free NEBNext NGS Library Prep sample!


Fields marked * are required.







Your institutional address:














The NEB eNews newsletter appears monthly and on special occasions. You will receive

• Technical tipps
• Exclusive promotions
• Information on new products and publications
• and much more...

You can unsubscribe at any time. Your complete information on data protection, in accordance with the requirements of the GDPR,


Show form

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.