NEBNext RNA Depletion

NEBNext rRNA & Globin mRNA Depletion Kits

NEBNext® rRNA Depletion Kit

The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) enables efficient removal of ribosomal RNA (rRNA) from total RNA for human, mouse and rat RNA samples.

This kit works well for both low-quality, degraded RNA (including FFPE RNA) and high-quality, intact RNA. rRNA depletion with a broad range of input amounts (10 ng to 1 μg) is completed in approximately 2 hours, with less than 10 minutes of hands-on time.

Advantages:
  • Suitable for FFPE (degraded) or high-quality (intact) RNA
  • 10 ng to 1 μg input
  • Remove > 95% of rRNA, and obtain more relevant sequence reads from your sample
  • Obtain a more complete transcriptome picture through retention of noncoding & incomplete RNAs that are lost with oligo d(T) poly(A) mRNA enrichment methods
  • Suitable for use with human, mouse or rat samples
  • Easily integrated upstream of any downstream random-primed cDNA synthesis protocol
  • Also available with RNA Sample Purification Beads

Application Note: Selective depletion of abundant RNAs to enable transcriptome analysis of low-input and highly-degraded RNA from FFPE breast cancer samples

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NEBNext rRNA Depletion Kit Workflow
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NEB_NEBNext rRNA Depletion Workflow

Neu: NEBNext® Globin & rRNA Depletion Kit

Globin mRNA and rRNA are highly abundant in blood samples, and their efficient and specific removal is desirable in order to reveal the biological significance of less abundant transcripts.

The NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete globin mRNA, cytoplasmic rRNA and mitochondrial rRNA with human, mouse and rat samples, and is effective with intact and degraded RNA.

Advantages:
  • Efficient, specific depletion of globin mRNA and rRNA
  • Suitable for low-quality or high-quality RNA
  • Compatible with a broad range of input amounts: 10 ng - 1 μg
  • Optional integration with poly(A) mRNA isolation workflows for removal of globin RNAs, rRNAs, and noncoding RNAs
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time
  • Also available with RNA Sample Purification Beads

Available products:

PRODUCT Prod. # SIZE PRICE
NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) E6310S 6 rxns 330 € In den Shop wechseln Produktinformationen
E6310L 24 rxns 1.200 € In den Shop wechseln Produktinformationen
E6310X 96 rxns 4.320 € In den Shop wechseln Produktinformationen
NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with Beads E6350S 6 rxns 374 € In den Shop wechseln Produktinformationen
E6350L 24 rxns 1.375 € In den Shop wechseln Produktinformationen
E6350X 96 rxns 4.950 € In den Shop wechseln Produktinformationen
NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) E7750S 6 rxns 340 € In den Shop wechseln Produktinformationen
E7750L 24 rxns 1.235 € In den Shop wechseln Produktinformationen
E7750X 96 rxns 4.450 € In den Shop wechseln Produktinformationen
NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads E7755S 6 rxns 350 € In den Shop wechseln Produktinformationen
E7755L 24 rxns 1.290 € In den Shop wechseln Produktinformationen
E7755X 96 rxns 4.635 € In den Shop wechseln Produktinformationen
You may also be interested in:
NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina E7760S 24 rxns 1.028 € In den Shop wechseln Produktinformationen
E7760L 96 rxns 3.495 € In den Shop wechseln Produktinformationen
NEBNext® Ultra II RNA Library Prep Kit for Illumina® E7770S 24 rxns 980 In den Shop wechseln Produktinformationen
E7770L 96 rxns 3.325 € In den Shop wechseln Produktinformationen
NEBNext® Poly(A) mRNA Magnetic Isolation Module E7490S 24 rxns 67 € In den Shop wechseln Produktinformationen
E7490L 96 rxns 242 € In den Shop wechseln Produktinformationen

As of: 03.01.2019
NEBNext products are available at a permanent low price that can not be further discounted.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.