The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) enables efficient removal of ribosomal RNA (rRNA) from total RNA for human, mouse and rat RNA samples. This kit works well for both low-quality, degraded RNA (including FFPE RNA) and high-quality, intact RNA. rRNA depletion with a broad range of input amounts (10 ng to 1 μg) is completed in approximately 2 hours, with less than 10 minutes of hands-on time.
Advantages
- A single kit that performs reliably well for all of your RNA samples:
- FFPE (degraded) or high-quality (intact) RNA
- 10 ng to 1 μg input amounts
- Remove > 95% of rRNA, and obtain more relevant sequence reads from your sample
- Obtain a more complete transcriptome picture through retention of noncoding & incomplete RNAs that are lost with oligo d(T) poly(A) mRNA enrichment methods
- Suitable for use with human, mouse or rat samples
- Easily integrated upstream of any downstream random-primed cDNA synthesis protocol
- No more need to homebrew: A reliable & convenient NGS-validated kit for the “RNase H protocol” for rRNA depletion
Application Note: Selective depletion of abundant RNAs to enable transcriptome analysis of low-input and highly-degraded RNA from FFPE breast cancer samples
NEBNext rRNA Depletion Kit Workflow
Click to enlarge
Product table
| PRODUCT | Prod. # | SIZE | PRICE | ||
|---|---|---|---|---|---|
| NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) | E6310S | 6 rxns | 330 € |  |
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| E6310L | 24 rxns | 1.200 € | |
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| E6310X | 96 rxns | 4.320 € | |
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| NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with Beads | E6350S | 6 rxns | 374 € | |
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| E6350L | 24 rxns | 1.375 € | |
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| E6350X | 96 rxns | 4.950 € | |
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| Passend dazu: | |||||
| NEBNext® Ultra Directional RNA Library Prep Kit for Illumina | E7420S | 24 rxns | 1.172 € | |
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| E7420L | 96 rxns | 3.801 € | |
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| NEBNext® Ultra RNA Library Prep Kit for Illumina® | E7530S | 24 rxns | 1.071 € | |
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| E7530L | 96 rxns | 3.425 € | |
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| NEBNext® Poly(A) mRNA Magnetic Isolation Module | E7490S | 24 rxns | 67 € | |
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| E7490L | 96 rxns | 242 € | |
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As of: 03.01.2019
NEBNext products are available at a permanent low price that can not be further discounted.
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
