NEBNext RNA Depletion

NEBNext rRNA Depletion Kit

The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) enables efficient removal of ribosomal RNA (rRNA) from total RNA for human, mouse and rat RNA samples. This kit works well for both low-quality, degraded RNA (including FFPE RNA) and high-quality, intact RNA. rRNA depletion with a broad range of input amounts (10 ng to 1 μg) is completed in approximately 2 hours, with less than 10 minutes of hands-on time.

Advantages

  • A single kit that performs reliably well for all of your RNA samples:
    • FFPE (degraded) or high-quality (intact) RNA
    • 10 ng to 1 μg input amounts
  • Remove > 95% of rRNA, and obtain more relevant sequence reads from your sample
  • Obtain a more complete transcriptome picture through retention of noncoding & incomplete RNAs that are lost with oligo d(T) poly(A) mRNA enrichment methods
  • Suitable for use with human, mouse or rat samples
  • Easily integrated upstream of any downstream random-primed cDNA synthesis protocol
  • No more need to homebrew: A reliable & convenient NGS-validated kit for the “RNase H protocol” for rRNA depletion

Application Note: Selective depletion of abundant RNAs to enable transcriptome analysis of low-input and highly-degraded RNA from FFPE breast cancer samples

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NEBNext rRNA Depletion Kit Workflow
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NEB_NEBNext rRNA Depletion Workflow

Product table

PRODUCT Prod. # SIZE PRICE
NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) E6310S 6 rxns 330 € In den Shop wechseln Produktinformationen
E6310L 24 rxns 1.200 € In den Shop wechseln Produktinformationen
E6310X 96 rxns 4.320 € In den Shop wechseln Produktinformationen
NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with Beads E6350S 6 rxns 374 € In den Shop wechseln Produktinformationen
E6350L 24 rxns 1.375 € In den Shop wechseln Produktinformationen
E6350X 96 rxns 4.950 € In den Shop wechseln Produktinformationen
Passend dazu:
NEBNext® Ultra Directional RNA Library Prep Kit for Illumina E7420S 24 rxns 1.172 € In den Shop wechseln Produktinformationen
E7420L 96 rxns 3.801 € In den Shop wechseln Produktinformationen
NEBNext® Ultra RNA Library Prep Kit for Illumina® E7530S 24 rxns 1.071 € In den Shop wechseln Produktinformationen
E7530L 96 rxns 3.425 € In den Shop wechseln Produktinformationen
NEBNext® Poly(A) mRNA Magnetic Isolation Module E7490S 24 rxns 67 € In den Shop wechseln Produktinformationen
E7490L 96 rxns 242 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

NEBNext products are available at a permanent low price that can not be further discounted.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.