As sequencing instrument capacity is increasing, and the ever-growing range of sequencing applications is demanding expanded sample preparation throughputs, many NGS users are turning to automation. Manual preparation and quantitation of large numbers of libraries can result in a sequencing workflow bottleneck, and automation of these protocols is an important, and increasingly popular, option.
Advantages of automation include:
- Increased throughput
- Improved reproducibility
- Increased speed
- Reduced hands-on time
- Reduced Repetitive Strain Injury (RSI) incidence
NEBNext® products are developed with automation in mind:
- Fewer components simplify automation setup
- Streamlined workflows minimize tip consumption and chances for pipetting errors
- Less clean-up bead consumption results in a lower cost-per-reaction
- Low input amount capability (ng for DNA/RNA, pg for ChIP) improves sequencing results with less sample
- Robust reagents improve overall library yields
- Sufficient kit component fill volumes for automation protocols improves usability
NEBNext kits have been automated by multiple automation platform providers, including Beckman Coulter®, Hamilton® and PerkinElmer®.
„NEB is currently the only company, which is offering really ``automation friendly`` library preparation kits. The volumes of components are calculated to cover for unavoidable deadvolumes, reactant volumes are in range of most automated platforms. The stability of the NEBNext chemistry allows a broad range of automation strategies.“
Dr. Jürgen Zimmermann, Senior Engineer - Automation in GeneCore, EMBL Heidelberg, Deutschland
Learn how Biomek automation of NEBNext RNA library preparation enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Fiona Stewart from New England Biolabs and Zach Smith from Beckman Coulter describe strand-specific RNA library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and discuss Biomek automation of this method.
Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Joanna Hamilton from Dartmouth Genomics & Molecular Biology Shared Resource, Lynne Apone from New England Biolabs and Zach Smith from Beckman Coulter describe the NEBNext Fast Fragmentation & DNA Library Prep kit for Ion Torrent, automation of this method and implementation in multiple workflows in a sequencing core lab.
E. coli K12 gDNA control libraries constructed with the NEBNext Ultra DNA automated method on the Biomek® platform
Traces from the Agilent® 2200 TapeStation, using D1000 ScreenTape, of six E. coli K12 control libraries show consistent performance. For more information see the Application Note Automated DNA Library Construction Using the NEBNext Ultra DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler
Checker Board Test for Cross-Contamination with the Hamilton STARlet™ platform
Bioanalyzer DNA 1000 traces from wells A1-A6 of the checker board test using E. coli DNA samples and water samples. The representative data of a 48 sample run reveal no cross contamination between individual wells. For more information see the Application Note Automated Library Preparation using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (E7370) on the HAMILTON STAR Line
Application Notes on Automation for download:
Beckman Coulter Life Sciences:
Automated DNA Library Construction Using the NEBNext Ultra DNA Library Prep Kit for Illumina on the Beckman Coulter Biomek FXP Automated Liquid Handler
Beckman Coulter Life Sciences:
Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler
Automated library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina on the epMotion 5075t
Automated Library Preparation using NEBNext Ultra DNA Library Prep Kit for Illumina (E7370) on the HAMILTON STAR Line
Automated size selection of NEBNext Small RNA libraries with the Sage Pippin Prep
Automated NEBNext Ultra II DNA Library Preparation for Illumina