NEBNext Ultra II RNA Library Prep Kit and Modules

Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA? To address these challenges, our next generation of RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries, enabling use of lower input amounts and fewer PCR cycles.

Our new NEBNext Ultra II RNA kits have streamlined, automatable workflows and are available for directional (strand-specific, using the “dUTP method”) and non-directional library prep, with the option of SPRIselect® beads for size-selection and clean-up steps.

Advantages:
  • Highest library yields
  • Generate high quality libraries even when you have only limited amounts of input RNA (10 ng – 1 µg Total RNA using the polyA mRNA workflow, 5 ng – 1 µg Total RNA using the rRNA depletion workflow)
  • Minimize bias, with fewer PCR cycles required
  • Directional and non-directional workflow options
  • Compatible with rRNA Depletion and poly(A) mRNA isolation reagents
  • Adaptors and primers for multiplexing (12- and 96-index) formats
  • Available with or without SPRIselect beads
  • Streamlined workflows, reduced hands-on time, and automation compatibility
  • Robust performance, even with low quality RNA

Request a free NEBNext Ultra II RNA Library Prep sample!

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UltraII_Dir_RNA_yield

Poly(A)-containing mRNA was isolated from 10 ng, 100 ng and 1 µg of Universal Human Reference RNA (Agilent #740000) and libraries were made using the NEBNext Ultra II Directional RNA kit, Kapa Stranded mRNA-Seq kit, Kapa mRNA HyperPrep kit and Illumina TruSeq Stranded mRNA Kit. The input RNA amount and number of PCR cycles are indicated. Library yields from an average of three replicates are shown. Error bars indicate standard deviation. Library yields were assessed using the Agilent® Bioanalyzer®.

NEBNext Ultra II Products are automation compatible and have been implemented on a number of platforms.

Available NEBNext Ultra II RNA Library Prep products

PRODUCT Prod. # SIZE PRICE
NEBNext® Ultra™ II Directional RNA Library Prep Kit Illumina E7760S 24 rxns 1.028 € In den Shop wechseln Produktinformationen
E7760L 96 rxns 3.495 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II Directional RNA Library Prep with Beads E7765S 24 rxns 1.140 € In den Shop wechseln Produktinformationen
E7765L 96 rxns 3.880 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® E7770S 24 rxns 980 € In den Shop wechseln Produktinformationen
E7770L 96 rxns 3.325 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II RNA Library Prep with Purification Beads E7775S 24 rxns 1.080 € In den Shop wechseln Produktinformationen
E7775L 96 rxns 3.685 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

NEBNext products are available at a permanent low price that can not be further discounted.

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This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

KAPA® is a registered trademark of Kapa Biosystems
ILLUMINA® and TRUSEQ® are registered trademarks of Illumina, Inc.
AGILENT® and BIOANALYZER® are registered trademarks of Agilent Technologies
SPRISELECT® is a registered trademark of Beckman Coulter, Inc.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.