Covid

NEBNext ARTIC Kits for SARS-CoV-2 Sequencing

The NEBNext ARTIC kits meet the increasing need for reliable, accurate and fast methods for sequencing SARSCoV-2, with Oxford Nanopore Technologies or Illumina sequencing. The kits use the ARTIC multiplexed ampliconbased whole-viral-genome sequencing approach and were developed in collaboration with the ARTIC Network (artic. network). The kits include balanced V3 primer pools for improved uniformity of genome coverage and are effective with 10-10,000 SARS-CoV-2 genome copies. The reagents and protocols for RT-PCR and library prep are all optimized specifically for the SARS-CoV-2 ARTIC workflows.

The two kits compatible with Illumina sequencing generate library inserts of ~150 bp (for 2 x 75 sequencing) or ~400 bp (for 2 x 250 sequencing), and include a novel DNA polymerase formulation that eliminates the need to normalize amplicon concentrations prior to library preparation.

Advantages:
  • Improved uniformity of SARS-CoV-2 genome coverage
  • Effective with a wide range of viral genome inputs (10–10,000 copies)
  • Single RT conditions for all input amounts
  • No amplicon normalization prior to library preparation (Illumina-compatible kits)
  • NEBNext Sample Purification Beads (SPRIselect®) provided
  • Cost-effective

NEBNext ARTIC Kit Options:

ARTIC Kit Options

NEBNext ARTIC Kit Workflow:

ARTIC Workflow

NEBNext ARTIC Kit Genome Coverage:

ARTIC Genome Coverage

Integrative Genome Viewer visualization of read coverage across the SARS-CoV-2 genome show the superior genome coverage and uniformity using the NEBNext primers, reagents and protocols. For detailled experimental data, please visit www.neb.com/E7660

„We have been impressed by the great performance of the NEBNext ARTIC FS kit in our sequencing of human samples containing SARS-CoV-2 RNA. With this kit we have been successfully processing samples of varying quality and quantity and have been amazed by its sensitivity and remarkable uniformity of the viral genome coverage. The quick turnaround time of the robust workflow has been truly beneficial for us.“

Dr. Vladimir Benes, Head of GeneCore EMBL Heidelberg, Early-Access-User

Questions regarding our NEBNext ARTIC SARS-CoV-2 kits?
We are happy to help you with setting up your protocols.
Please contact our application specialists:

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Product table

PRODUCT Prod. # SIZE PRICE
NEBNext ARTIC SARS-CoV-2 Library Prep Kit (Illumina) E7650S 24 rnx 384 € NEB Shop_icon NEB Info_icon
NEBNext ARTIC SARS-CoV-2 Library Prep Kit (Illumina) E7650L 96 rnx 1536 € NEB Shop_icon NEB Info_icon
NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) E7658S 24 rnx 408 € NEB Shop_icon NEB Info_icon
NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) E7658L 96 rnx 1632 € NEB Shop_icon NEB Info_icon
NEBNext ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies) E7660S 24 rnx 192 € NEB Shop_icon NEB Info_icon
NEBNext ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies) E7660L 96 rnx 768 € NEB Shop_icon NEB Info_icon
NEBNext ARTIC SARS-CoV-2 RT-PCR Module E7626S 24 rnx 78 € NEB Shop_icon NEB Info_icon
NEBNext ARTIC SARS-CoV-2 RT-PCR Module E7626L 96 rnx 310 € NEB Shop_icon NEB Info_icon

As of: 14.05.2021

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.