The NEBNext ARTIC kits meet the increasing need for reliable, accurate and fast methods for sequencing SARSCoV-2, with Oxford Nanopore Technologies or Illumina sequencing. The kits use the ARTIC multiplexed ampliconbased whole-viral-genome sequencing approach and were developed in collaboration with the ARTIC Network (artic. network). The kits include balanced V3 primer pools for improved uniformity of genome coverage and are effective with 10-10,000 SARS-CoV-2 genome copies. The reagents and protocols for RT-PCR and library prep are all optimized specifically for the SARS-CoV-2 ARTIC workflows.
The two kits compatible with Illumina sequencing generate library inserts of ~150 bp (for 2 x 75 sequencing) or ~400 bp (for 2 x 250 sequencing), and include a novel DNA polymerase formulation that eliminates the need to normalize amplicon concentrations prior to library preparation.
- Improved uniformity of SARS-CoV-2 genome coverage
- Effective with a wide range of viral genome inputs (10–10,000 copies)
- Single RT conditions for all input amounts
- No amplicon normalization prior to library preparation (Illumina-compatible kits)
- NEBNext Sample Purification Beads (SPRIselect®) provided
NEBNext ARTIC Kit Options:
NEBNext ARTIC Kit Workflow:
NEBNext ARTIC Kit Genome Coverage:
Integrative Genome Viewer visualization of read coverage across the SARS-CoV-2 genome show the superior genome coverage and uniformity using the NEBNext primers, reagents and protocols. For detailled experimental data, please visit www.neb.com/E7660
„We have been impressed by the great performance of the NEBNext ARTIC FS kit in our sequencing of human samples containing SARS-CoV-2 RNA. With this kit we have been successfully processing samples of varying quality and quantity and have been amazed by its sensitivity and remarkable uniformity of the viral genome coverage. The quick turnaround time of the robust workflow has been truly beneficial for us.“
Dr. Vladimir Benes, Head of GeneCore EMBL Heidelberg, Early-Access-User
As of: 14.05.2021