Archiving of clinical materials as Formalin-Fixed, Paraffin-Embedded (FFPE) samples is a common practice. However, the methods used for fixation and storage significantly damage and compromise the quality of nucleic acids from these samples. As a result, it can be challenging to obtain useful information, including high-quality sequence data, especially when sample amounts are limited. The NEBNext FFPE DNA Repair Mix is a cocktail of enzymes formulated to repair DNA, and specifically optimized and validated for repair of FFPE DNA samples.
- Reliable and easy protocol
- Improves library quality through repair of damages from FFPE fixation
- No change/bias compared to untreated sequence
- Application directly prior to the Library Prep step, therefore compatible with all platforms and workflows