FFPE DNA Repair Mix

NEBNext FFPE DNA Repair Mix

Archiving of clinical materials as Formalin-Fixed, Paraffin-Embedded (FFPE) samples is a common practice. However, the methods used for fixation and storage significantly damage and compromise the quality of nucleic acids from these samples. As a result, it can be challenging to obtain useful information, including high-quality sequence data, especially when sample amounts are limited. The NEBNext FFPE DNA Repair Mix is a cocktail of enzymes formulated to repair DNA, and specifically optimized and validated for repair of FFPE DNA samples.

Advantages:
  • Reliable and easy protocol
  • Improves library quality through repair of damages from FFPE fixation
  • No change/bias compared to untreated sequence
  • Application directly prior to the Library Prep step, therefore compatible with all platforms and workflows

Figure: The effect of DNA repair on the percentage of mappable reads. Reads were mapped to the human genome (hg19) using BWA (1).

FFPE Mapped Reads

Figure: An example of Agilent Bioanalyzer® traces of libraries prepared from stomach tumor FFPE DNA that was treated with the FFPE DNA Repair Mix, or was untreated, before library construction. Yield improvements of 10% to 458% have been observed. 

FFPE DNA Repair Mix

Product table

PRODUCT Prod. # SIZE PRICE
NEBNext® FFPE DNA Repair Mix M6630S 24 rxns 173 € In den Shop wechseln Produktinformationen
M6630L 96 rxns 604 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

NEBNext products are available at a permanent low price that can not be further discounted.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.