Archiving of clinical materials as Formalin-Fixed, Paraffin-Embedded (FFPE) samples is a common practice. However, the methods used for fixation and storage significantly damage and compromise the quality of nucleic acids from these samples. As a result, it can be challenging to obtain useful information, including high-quality sequence data, especially when sample amounts are limited. The NEBNext FFPE DNA Repair Mix is a cocktail of enzymes formulated to repair DNA, and specifically optimized and validated for repair of FFPE DNA samples.
- Reliable and easy protocol
- Improves library quality through repair of damages from FFPE fixation
- No change/bias compared to untreated sequence
- Application directly prior to the Library Prep step, therefore compatible with all platforms and workflows
Figure: The effect of DNA repair on the percentage of mappable reads. Reads were mapped to the human genome (hg19) using BWA (1).
Figure: An example of Agilent Bioanalyzer® traces of libraries prepared from stomach tumor FFPE DNA that was treated with the FFPE DNA Repair Mix, or was untreated, before library construction. Yield improvements of 10% to 458% have been observed.