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Q5 High Fidelity DNA Polymerase

A new standard for High Fidelity PCR

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance.

With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates, even lower than Phusion Polymerase

Engineered for Performance

Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer.

Video: Q5 High-Fidelity Polymerase

Q5 DNA Polymerase is also available as a hot start version. The Q5 Hot Start DNA Polymerase utilizes NEBs aptamer technology, allowing room temperature reaction setup without the need of an additional activation step.

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Five Quality Features of Q5

Q1 Fidelity

The highest fidelity amplification available (~280X higher than Taq, ~7x higher than Phusion Polymerase)”Ultra-Fidelity” (~280X higher than Taq, ~7x higher than Phusion Polymerase)

Q2 Robustness

High specificity and yield with minimal optimization

Q3 Coverage

Superior performance for a broad range of amplicons (from high AT to high GC)

Q4 Speed

Short extension times

Q5 Amplicon length

Robust amplifications up to 20 kb for simple templates, and 10 kb for complex

Video: Important tips for Q5 Polymerase

Feature Article:

„Polymerase Fidelity –
What is it and what does it mean for your PCR?“

Q5 DNA Polymerase Fidelity Brochure
Download PDF

NEU: Q5U Hot Start High-Fidelity DNA Polymerase

Q5U is a modified version of Q5 High-Fidelity DNA Polymerase containing a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

This is useful for amplifying bisulfite-converted, enzymatically-deaminated, or damaged DNA, preventing carryover contamination in PCR (when used with dUTP and UDG), and in USER cloning methods.

Optimized protocols are available for recommended applications.

Q5U High Fidelity Polymerase Applications
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Comparison of High-Fidelity Polymerases

Q5 DNA Polymerase Comparison Table

1 We continue to investigate improved assays to characterize Q5’s very low error rate to ensure thatwe present the most accurate fidelity data possible (Potapov, V. and Ong, J.L. (2017) PLoS ONE.12(1): e0169774.).
2 PCR-based mutation screening in lacZ (NEB), lacI (Agilent) or rpsL (Life)
3 Template not specified.
4 Simple templates include plasmid, viral and E. coli genomic DNA. Complex templates include plant, human and other mammalian genomic DNA.
5 Values provided by individual manufacturers.

Amplification Performance Across a Wide Range of Genomic Targets

Q5 DNA Polymerase performance on genomic targets

PCR was performed with a variety of amplicons, with GC content ranging from high AT to high GC, with Q5 and several other commercially available polymerases. All polymerases were cycled according to manufacturer’s recommendations, including use of GC Buffers and enhancers when recommended. Yield and purity of reaction products were quantitated and represented, as shown in the figure key, by dot color and size. A large dark green dot represents the most successful performance. Q5 provides superior performance across the range of GC content.

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Available Q5 Polymerase Products

PRODUCT Prod. # Size PRICE
Q5 High-Fidelity DNA Polymerase M0491S 100 units 96 € In den Shop wechseln Produktinformationen
M0491L 500 units 424 € In den Shop wechseln Produktinformationen
Q5 High-Fidelity 2X Master Mix M0492S 100 rxns (50 µl/vol) 157 € In den Shop wechseln Produktinformationen
M0492L 500 rxns (50 µl/vol) 628 € In den Shop wechseln Produktinformationen
Q5 Hot Start High-Fidelity DNA Polymerase M0493S 100 units 112 € In den Shop wechseln Produktinformationen
M0493L 500 units 508 € In den Shop wechseln Produktinformationen
Q5 Hot Start High-Fidelity 2X Master Mix M0494S 100 rxns (50 µl/vol) 180 € In den Shop wechseln Produktinformationen
M0494L 500 rxns (50 µl/vol) 720 € In den Shop wechseln Produktinformationen
Q5U Hot Start High-FidelityDNA Polymerase M0515S 100 units 152 € In den Shop wechseln Produktinformationen
M0515L 500 units 640 € In den Shop wechseln Produktinformationen
Also available as a PCR Kit:
Q5 High-Fidelity PCR Kit E0555S 50 rxns 92 € In den Shop wechseln Produktinformationen
E0555L 200 rxns 312 € In den Shop wechseln Produktinformationen
Optimized for Next-Gen-Seq library prep:
NEBNext High-Fidelity 2X PCR Master Mix M0541S 50 rxns (50 µl/vol) 90 € In den Shop wechseln Produktinformationen
M0541L 250 rxns (50 µl/vol) 360 € In den Shop wechseln Produktinformationen
NEBNext Q5U Master Mix M0597S 50 rxns (50 µl/vol) 125 € In den Shop wechseln Produktinformationen
M0597L 250 rxns (50 µl/vol) 495 € In den Shop wechseln Produktinformationen
Companion products:
Deoxynucleotide Solutions, Mix N0447S 8 µmol of each 61 € In den Shop wechseln Produktinformationen
N0447L 40 µmol of each 244 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

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