Q5 High Fidelity DNA Polymerase

A new standard for High Fidelity PCR

Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance.

With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates, even lower than Phusion Polymerase

Engineered for Performance

Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer.

Q5 DNA Polymerase is also available as a hot start version. The Q5 Hot Start DNA Polymerase utilizes NEBs aptamer technology, allowing room temperature reaction setup without the need of an additional activation step.

Video: Q5 High-Fidelity Polymerase

Q5 DNA Polymerase is also available as a hot start version. The Q5 Hot Start DNA Polymerase utilizes NEBs aptamer technology, allowing room temperature reaction setup without the need of an additional activation step.

Get a free Sample of Q5 High Fidelity Polymerase!

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Five Quality Features of Q5

Q1 Fidelity

The highest fidelity amplification available (~280X higher than Taq, ~7x higher than Phusion Polymerase)”Ultra-Fidelity” (~280X higher than Taq, ~7x higher than Phusion Polymerase)

Q2 Robustness

High specificity and yield with minimal optimization

Q3 Coverage

Superior performance for a broad range of amplicons (from high AT to high GC)

Q4 Speed

Short extension times

Q5 Amplicon length

Robust amplifications up to 20 kb for simple templates, and 10 kb for complex

Video: Important tips for Q5 Polymerase

Feature Article:

„Polymerase Fidelity –
What is it and what does it mean for your PCR?“

Polymerase-Fidelity--What-is-it-and-what-does-it-mean-for-your-PCR
Download PDF

Comparison of High-Fidelity Polymerases

1 We continue to investigate improved assays to characterize Q5’s very low error rate to ensure thatwe present the most accurate fidelity data possible (Potapov, V. and Ong, J.L. (2017) PLoS ONE.12(1): e0169774.).
2 PCR-based mutation screening in lacZ (NEB), lacI (Agilent) or rpsL (Life)
3 Template not specified.
4 Simple templates include plasmid, viral and E. coli genomic DNA. Complex templates include plant, human and other mammalian genomic DNA.
5 Values provided by individual manufacturers.

Amplification Performance Across a Wide Range of Genomic Targets

PCR was performed with a variety of amplicons, with GC content ranging from high AT to high GC, with Q5 and several other commercially available polymerases. All polymerases were cycled according to manufacturer’s recommendations, including use of GC Buffers and enhancers when recommended. Yield and purity of reaction products were quantitated and represented, as shown in the figure key, by dot color and size. A large dark green dot represents the most successful performance. Q5 provides superior performance across the range of GC content.

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PCR

Recommended products:

  • Q5 High-Fidelity DNAPolymerase (NEB #M0491)
  • Q5 Hot Start High-Fidelity DNA Polymerase (NEB #M0493)
  • Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515)
  • Q5 High-Fidelity 2X Master Mix (NEB #M0492)
  • Q5 Hot Start High-Fidelity 2X Master Mix (NEB #M0494)
  • Q5 High-Fidelity PCR Kit (NEB #E0555)
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NGS LIBRARY AMPLIFICATION

Recommended products:

  • NEBNext Ultra II Q5 Master Mix (NEB #M0544)
  • NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)
  • NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB #M0543)
  • NEBNext Q5U Master Mix (NEB #M0597)
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ARTIC VIRAL SEQUENCING

Recommended products:

One-Step

  • LunaScript Multiplex One-Step RT-PCR Kit (NEB #E1555)

Two-Step

  • LunaScript RT SuperMix (NEB #M3010)
  • Q5 Hot Start High- Fidelity 2X Master Mix (NEB #M0494)
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DIRECT SAMPLE AMPLIFICATION

Recommended products:

  • Q5 Blood Direct 2X Master Mix (NEB #M0500)
Icon_Mutagenese

MUTAGENESIS

Recommended products:

  • Q5 Site-Directed Mutagenesis Kit (NEB #E0554)
  • Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) (NEB #E0552)

Available Q5 Polymerase Products

PRODUCT Prod. # Size PRICE
Q5 High-Fidelity DNA Polymerase M0491S 100 units 101 € In den Shop wechseln Produktinformationen
M0491L 500 units 444 € In den Shop wechseln Produktinformationen
Q5 High-Fidelity 2X Master Mix M0492S 100 rxns (50 µl/vol) 167 € In den Shop wechseln Produktinformationen
M0492L 500 rxns (50 µl/vol) 668 € In den Shop wechseln Produktinformationen
Q5 Hot Start High-Fidelity DNA Polymerase M0493S 100 units 118 € In den Shop wechseln Produktinformationen
M0493L 500 units 534 € In den Shop wechseln Produktinformationen
Q5 Hot Start High-Fidelity 2X Master Mix M0494S 100 rxns (2×1.25ml) 192 € In den Shop wechseln Produktinformationen
M0494L 500 rxns (10×1.25ml) 768 € In den Shop wechseln Produktinformationen
M0494X 500 rxns (1×12.5ml) 766 € In den Shop wechseln Produktinformationen
Q5U Hot Start High-FidelityDNA Polymerase M0515S 100 units 158 € In den Shop wechseln Produktinformationen
M0515L 500 units 666 € In den Shop wechseln Produktinformationen
Also available as Q5 High-Fidelity PCR Kit:
Q5 High-Fidelity PCR Kit E0555S 50 rxns 98 € In den Shop wechseln Produktinformationen
E0555L 200 rxns 333 € In den Shop wechseln Produktinformationen
Also available as Next-Gen-Seq optimized formula:
NEBNext Ultra II Q5 Master Mix M0544S 50 rxns (50 µl vol) 99 € In den Shop wechseln Produktinformationen
M0544L 250 rxns (50 µl vol) 395 € In den Shop wechseln Produktinformationen
M0544X 500 rxns (50 µl vol) 751 € In den Shop wechseln Produktinformationen
NEBNext High-Fidelity 2X PCR Master Mix M0541S 50 rxns (50 µl/vol) 90 € In den Shop wechseln Produktinformationen
M0541L 250 rxns (50 µl/vol) 360 € In den Shop wechseln Produktinformationen
NEBNext Q5 Hot Start HiFi PCR Master Mix M0543S 50 rxns (50 µl vol) 90 € In den Shop wechseln Produktinformationen
M0543L 250 rxns (50 µl vol) 360 € In den Shop wechseln Produktinformationen
NEBNext Q5U Master Mix M0597S 50 rxns (50 µl/vol) 125 € In den Shop wechseln Produktinformationen
M0597L 250 rxns (50 µl/vol) 495 € In den Shop wechseln Produktinformationen
or for “ARTIC” similar workflows:
One-Step:
LunaScript Multiplex One-Step RT-PCR Kit
E1555S 50 rxns 225 € In den Shop wechseln Produktinformationen
E1555L 250 rxns 875 € In den Shop wechseln Produktinformationen
Two-Step:
LunaScript RT SuperMix
M3010L 100 rxns 403 € In den Shop wechseln Produktinformationen
M3010X 500 rxns 1.612 € In den Shop wechseln Produktinformationen
M3010E 2500 rxns 7.053 € In den Shop wechseln Produktinformationen
Also available as Direct Sample Amplification formula:
Q5 Blood Direct 2X Master Mix M0500S 100 rxns (1x1ml) 107 € In den Shop wechseln Produktinformationen
M0500L 500 rxns (5x1ml) 465 € In den Shop wechseln Produktinformationen
Also available for Mutagenesis:
Q5 Site-Directed Mutagenesis Kit E0554S 10 rxns 204 € In den Shop wechseln Produktinformationen
Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) E0552S 10 rxns 140 € In den Shop wechseln Produktinformationen
Corresponding products:
Deoxynucleotide Solutions, Mix N0447S 8 µmol of each 64 € In den Shop wechseln Produktinformationen
N0447L 40 µmol of each 255 € In den Shop wechseln Produktinformationen

As of: 22.09.2022

Order a free Q5 HiFi DNA Polymerase sample:

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This offer is limited to new customers and to 1 sample per working group. Samples are available as long as supplies last.

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