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Isothermal Amplification – Polymerases

Bst 2.0 DNA Polymerase

Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment). Bst 2.0 DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.

Bst 2.0 WarmStart™ DNA Polymerase

Bst 2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. The aptamer rapidly releases the Bst 2.0 WarmStart DNA Polymerase above 45°C and therefore no special activation step is needed to activate the polymerase.

Cover-FeatureArticle_ImprovedIsoAmpReagents

„Improved Reagents for Isothermal DNA Amplification“

Dr. Nathan Tanner, New England Biolabs Inc.

Advantages:

  • “engineered for performance”
  • Faster
  • Increased thermostability
  • Improved salt tolerance
  • Higher yields
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New: Bst 3.0 DNA Polymerase – optimal for LAMP and RT-LAMP

Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity.

Bst 3.0 DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity.

Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.

– in short: „engineered for performance!“

The superior properties of Bst 3.0 DNA Polymerase make it a first choice enzyme for all of your (RT)-LAMP Experiments.

Fast, single-enzyme RT-LAMP can be performed using Bst 3.0 RT-LAMP was performed using indicated DNA polymerase and Jurkat total RNA and primers for two genes (ACTB, left; HMBS2, right). Fastest results were observed with a 2-enzyme system, Bst 2.0 and WarmStart RTx, but robust amplification was also observed using Bst 3.0 without additional RT. Bst LF, Bst 2.0 and competitor enzymes showed highly variable performance, with slow threshold times or reaction failure on one of the two targets.

Not sure which polymerase you need for
your Isothermal Amplification?

We have an overview and background information on Isothermal Amplification in our current brochure.

Available Products

Product table

PRODUCT Prod. # SIZE PRICE
Bst 2.0 DNA Polymerase M0537S 1.600 units 70 € In den Shop wechseln Produktinformationen
M0537L 8.000 units 280 € In den Shop wechseln Produktinformationen
Bst 2.0 DNA Polymerase, conc. M0537M 8.000 units 280 € In den Shop wechseln Produktinformationen
Bst 2.0 Warm Start DNA Polymerase M0538S 1.600 units 78 € In den Shop wechseln Produktinformationen
M0538L 8.000 units 312 In den Shop wechseln Produktinformationen
Bst 2.0 Warm Start DNA Polymerase, conc. M0538M 8.000 units 312 € In den Shop wechseln Produktinformationen
Bst 3.0 DNA Polymerase M0374S 1.600 units 71 € In den Shop wechseln Produktinformationen
M0374L 8.000 units 284 € In den Shop wechseln Produktinformationen
Bst 3.0 DNA Polymerase, conc. M0374M 8.000 units 284 € In den Shop wechseln Produktinformationen
Isothermal Amplification Buffer B0537S 6.0 ml 28 In den Shop wechseln Produktinformationen
Passend dazu:
Deoxynucleotide Solutions, Mix N0447S 8 µmol of each 62 € In den Shop wechseln Produktinformationen
N0447L 40 µmol of each 248 € In den Shop wechseln Produktinformationen

As of: 01.01.2020

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.