The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit allows for the real-time detection of SARS-CoV-2 nucleic acid* by RT-PCR. The kit contains primers and probes specific for two regions of the virus N gene. The probes are labeled with two different fluorophores (N1: HEX; N2: FAM) to enable simultaneous detection of both signals. Multiplex-detection enables high throughput workflows.
- Reduced background amplification from gDNA using a modified RNase P internal control reverse primer
- LUNA WarmStart technology allows room temperature setup
- Reduced risk of carryover contamination with thermolabile UDG and dUTP in the master mix
- Supports sample pooling with minimal loss in sensitivity
* Research Use Only
Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit components
Using the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, up to 94 different samples can be assessed in a single 96-well plate. Anticipated results for each sample type are shown (in each fluorophore channel).
The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit demonstrates a lower limit of detection than TaqPath™1-Step RT-qPCR Master Mix, CG
LOD comparison using: Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit for multiplex RT-qPCR targeting 2019-nCoV_N1 target (HEX) and 2019-nCoV_N2 target (FAM), according to reaction and cycling conditions provided in the E3019 product manual, and TaqPath 1-Step RT-qPCR Master Mix, CG for singleplex RT-qPCR targeting 2019-nCoV_ N1 (FAM) and 2019-nCoV_N2 (FAM), according to the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel guidelines. Performance was evaluated using Synthetic Twist SARS-CoV-2 RNA Control 2 diluted in 10 ng of Jurkat total RNA. Data was collected on an Applied Biosystems® 7500 Fast real-time instrument (96-well, 20 µl reactions). Under these conditions, the Luna Kit has an LOD of 5 copies/reaction for both targets while the LOD using TaqPath is 10 copies/reaction for these targets.