Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit

The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit allows for the real-time detection of SARS-CoV-2 nucleic acid* by RT-PCR. The kit contains primers and probes specific for two regions of the virus N gene. The probes are labeled with two different fluorophores (N1: HEX; N2: FAM) to enable simultaneous detection of both signals. Multiplex-detection enables high throughput workflows.

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Advantages:
  • Reduced background amplification from gDNA using a modified RNase P internal control reverse primer
  • LUNA WarmStart technology allows room temperature setup
  • Reduced risk of carryover contamination with thermolabile UDG and dUTP in the master mix
  • Supports sample pooling with minimal loss in sensitivity

* Research Use Only

SARS-CoV2_RTqPCR_Components

Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit components

SARS-CoV2_RTqPCR_PosNeg

Using the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, up to 94 different samples can be assessed in a single 96-well plate. Anticipated results for each sample type are shown (in each fluorophore channel).

SARS-CoV2_RTqPCR_vsTaqPathLOD

The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit demonstrates a lower limit of detection than TaqPath™1-Step RT-qPCR Master Mix, CG

LOD comparison using: Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit for multiplex RT-qPCR targeting 2019-nCoV_N1 target (HEX) and 2019-nCoV_N2 target (FAM), according to reaction and cycling conditions provided in the E3019 product manual, and TaqPath 1-Step RT-qPCR Master Mix, CG for singleplex RT-qPCR targeting 2019-nCoV_ N1 (FAM) and 2019-nCoV_N2 (FAM), according to the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel guidelines. Performance was evaluated using Synthetic Twist SARS-CoV-2 RNA Control 2 diluted in 10 ng of Jurkat total RNA. Data was collected on an Applied Biosystems® 7500 Fast real-time instrument (96-well, 20 µl reactions). Under these conditions, the Luna Kit has an LOD of 5 copies/reaction for both targets while the LOD using TaqPath is 10 copies/reaction for these targets.

PRODUCT Prod.Nr.: SIZE PRICE
SARS-CoV-2 Positive Control (N gene) N2117S 50 µl 70 € NEB Shop_icon NEB Info_icon
Luna SARS-CoV-2 Multiplex Assay Kit E3019S 96 rxns 295 € NEB Shop_icon NEB Info_icon
Luna SARS-CoV-2 Multiplex Assay Kit E3019L 480 rxns 1288 € NEB Shop_icon NEB Info_icon

As of: 01.01.2021

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.