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Luna Cell Ready One-Step RT-qPCR Kits

Luna Cell Ready One-Step RT qPCR Kits – 

Go direct from cells to RNA quantitation without purification

The Luna Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification.

Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. The Luna Cell Ready One-Step RT-qPCR Kit offers an alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis.

Advantages:
  • Contains reagents for 100 lysis preps and 500 one-step RT-qPCR reactions (dye)
  • Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines
  • Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
  • Features Luna WarmStart RT paired with Hot Start Taq for increased thermostability and room temperature setup
  • Additional lysis preps available: Luna Cell Ready Lysis Module (NEB #E3032)

By coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple workflow resulting in effective cell lysis, RNA release and genomic DNA removal simultaneously in a 15-minute protocol. Up to 2 µl lysate (equivalent to RNA from 0.2 – 4,000 cells) can be transferred into 20 µl downstream RT-qPCR reactions.

The Luna Cell Ready One-Step RT-qPCR Kit offers reliable and precise RNA quantitation comparable to purified RNA across 5-log cell inputs.

Detection of β-actin, an abundant target, and ARF3 and Tubulin, two less abundant targets, across 5-logs of cell inputs. Efficiency (E) for each target is shown at the lower left corners of the panel. B. Cq relative to β-actin at each dilution (ΔCq (GOI)) was calculated from the data in (A) across 5-log cell inputs. The average Cqs are shown as bars

The new Luna Cell Ready One-Step RT-qPCR Kits make the quantification of RNA faster and easier than ever before.

PRODUCT Prod.Nr.: SIZE PRICE
Luna Cell Ready One-Step RT-qPCR Kit E3030S 100 + 500 rxns 898 € NEB Shop_icon NEB Info_icon
Luna Cell Ready Probe One-Step RT-qPCR Kit E3031S 100 + 500 rxns 854 € NEB Shop_icon NEB Info_icon
Luna Cell Ready Lysis Module E3032S 100 rxns 450 € NEB Shop_icon NEB Info_icon

As of: 01.01.2021

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.