Luna Cell Ready One-Step RT qPCR Kits –
Go direct from cells to RNA quantitation without purification
The Luna Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification.
Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. The Luna Cell Ready One-Step RT-qPCR Kit offers an alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis.
- Contains reagents for 100 lysis preps and 500 one-step RT-qPCR reactions (dye)
- Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines
- Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
- Features Luna WarmStart RT paired with Hot Start Taq for increased thermostability and room temperature setup
- Additional lysis preps available: Luna Cell Ready Lysis Module (NEB #E3032)
By coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple workflow resulting in effective cell lysis, RNA release and genomic DNA removal simultaneously in a 15-minute protocol. Up to 2 µl lysate (equivalent to RNA from 0.2 – 4,000 cells) can be transferred into 20 µl downstream RT-qPCR reactions.
The Luna Cell Ready One-Step RT-qPCR Kit offers reliable and precise RNA quantitation comparable to purified RNA across 5-log cell inputs.
Detection of β-actin, an abundant target, and ARF3 and Tubulin, two less abundant targets, across 5-logs of cell inputs. Efficiency (E) for each target is shown at the lower left corners of the panel. B. Cq relative to β-actin at each dilution (ΔCq (GOI)) was calculated from the data in (A) across 5-log cell inputs. The average Cqs are shown as bars
The new Luna Cell Ready One-Step RT-qPCR Kits make the quantification of RNA faster and easier than ever before.
|Luna Cell Ready One-Step RT-qPCR Kit||E3030S||100 + 500 rxns||932 €|
|Luna Cell Ready Probe One-Step RT-qPCR Kit||E3031S||100 + 500 rxns||886 €|
|Luna Cell Ready Lysis Module||E3032S||100 rxns||465 €|
As of: 03.01.2022