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Taq DNA Polymerases

Taq DNA Polymerase

Taq DNA Polymerase is commonly used for routine PCR applications in many molecular biology labs.

For your “daily” PCR we recommend NEBs reliable Taq DNA Polymerase, which is available in various Buffer- and Enzyme-formulations.

Advantages:
  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Robust and reliable reactions
  • Tolerates a wide range of templates
  • Incorporates dUTP, dITP and fluorescently-labeled nucleotides
  • Exceptional value in terms of cost per unit

Hot Start Taq DNA Polymerase

Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme.

NEB’s Hot Start Taq shows excellent yields while being highly specific

Manufacturer DNA Polymerase Hot Start Inhibitor Activation time** Performance*
Specificity Yield
Applied Biosystens AmpliTaq Gold 360 Chem 10 min NEB HSTaq_3 NEB HSTaq_3
Invitrogen Platinum Taq  Ab 30 sec NEB HSTaq_1 NEB HSTaq_2
NEB Hot Start Taq Aptamer Keine NEB HSTaq_4 NEB HSTaq_3
Promega GoTaq Hot Ab 2 min NEB HSTaq_1 NEB HSTaq_1
Qiagen Hot Star Taq Chem 15 min NEB HSTaq_1 NEB HSTaq_3
Roche FastStart Taq Chem 4 min NEB HSTaq_3 NEB HSTaq_4
Sigma JumpStart Taq Ab 1 min NEB HSTaq_3 NEB HSTaq_4
Thermo Scientific Maxima Hot Start Taq Chem 4 min NEB HSTaq_2 NEB HSTaq_2
Taq DNA Polymerase Legende

*Performance compared to all enzymes tested. Results are from triplicate reactions on a series of human genomic amplicons. Performance was scored for both yield and desired product. **Time required to activate hot start enzymes may include initiation denaturation time, if not specified.

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Product table

PRODUCT Prod. # SIZE PRICE
Taq DNA Polymerase M0267S 400 units 60 € In den Shop wechseln Produktinformationen
M0267L 2.000 units 240 € In den Shop wechseln Produktinformationen
M0267X 4.000 units 440 € In den Shop wechseln Produktinformationen
M0267E 20.000 units PRICE TIP: 1.200 € In den Shop wechseln Produktinformationen
Taq DNA Polymerase (DF Standard Taq Buffer) M0273S 400 units 60 € In den Shop wechseln Produktinformationen
M0273L 2.000 units 240 € In den Shop wechseln Produktinformationen
M0273X 4.000 units 440 € In den Shop wechseln Produktinformationen
M0273E 20.000 units PRICE TIP: 1.200 € In den Shop wechseln Produktinformationen
Taq 2X Master Mix M0270L 500 rxns 143 € In den Shop wechseln Produktinformationen
Quick-Load® Taq 2X Master Mix M0271L 500 rxns 220 € In den Shop wechseln Produktinformationen
Taq DNA Polymerase, Standard (Mg-free) Buffer M0320S 400 units 60 € In den Shop wechseln Produktinformationen
M0320L 2.000 units 240 € In den Shop wechseln Produktinformationen
Taq 5X Master Mix M0285L 500 rxns 143 € In den Shop wechseln Produktinformationen
Hot Start Taq 2X Master Mix M0496S 100 rxn (50 µl vol) 59 € In den Shop wechseln Produktinformationen
M0496L 500 rxn (50 µl vol) 236 € In den Shop wechseln Produktinformationen
Hot Start Taq DNA Polymerase (Standard Taq Buffer) M0495S 200 units 67 € In den Shop wechseln Produktinformationen
M0495L 1.000 units 268 € In den Shop wechseln Produktinformationen
Companion products:
Deoxynucleotide Solutions, Mix N0447S 8 µmol of each 62 € In den Shop wechseln Produktinformationen
N0447L 40 µmol of each 248 € In den Shop wechseln Produktinformationen

As of: 01.01.2020

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.