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Phusion DNA Polymerase

Phusion® Technology

Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA Polymerase, 7.5 kb genomic DNA and 20 kb λ DNA are routinely amplified. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning. The Phusion High-Fidelity DNA Polymerase sets a new standard for PCR performance.

Try
Q5® DNA Polymerase
Higher fidelity &
faster extension times
at the same price!

Advantages:
  • Extreme Fidelity (50X greater than Taq)
  • High Speed - Extension times are dramatically reduced (10X faster than Pfu)
  • Robust Reactions - Maximal success with minimal optimization
  • High Yield - Increased product yield with minimal enzyme amount

NEB_PHUSION_3

3.8 kb fragment was amplified from 50 ng of Jurkat gDNA using different polymerases. Reactions were carried out according to the manufacter’s recommended conditions (cycling conditions are shown below). Extension times are indicated (in minutes).. Ladder L is a 1 kb DNA Ladder #N3232.

NEB Tm Calculator:

Determine your primers’ optimal annealing temperature.

TmCalculator

Product table

PRODUCT Prod. # SIZE PRICE
Phusion® High-Fidelity DNA Polymerase M0530S 100 units 98 € In den Shop wechseln Produktinformationen
M0530L 500 units 432 € In den Shop wechseln Produktinformationen
Phusion® High-Fidelity PCR Master Mix with HF Buffer M0531S 100 rxns (50 µl/vol) 164 € In den Shop wechseln Produktinformationen
M0531L 500 rxns (50 µl/vol) 650 € In den Shop wechseln Produktinformationen
Phusion® High-Fidelity PCR Master Mix with GC Buffer M0532S 100 rxns (50 µl/vol) 164 € In den Shop wechseln Produktinformationen
M0532L 500 rxns (50 µl/vol) 650 € In den Shop wechseln Produktinformationen
Phusion® High-Fidelity PCR Kit E0553S 50 rxns 68 € In den Shop wechseln Produktinformationen
E0553L 200 rxns 238 € In den Shop wechseln Produktinformationen
Phusion Hot Start Flex Polymerases
Phusion® Hot Start Flex DNA Polymerase M0535S 100 units 114 € In den Shop wechseln Produktinformationen
M0535L 500 units 518 € In den Shop wechseln Produktinformationen
Phusion® Hot Start Flex 2X Master Mix M0536S 100 rxns (50 µl/vol) 185 € In den Shop wechseln Produktinformationen
M0536L 500 rxns (50 µl/vol) 735 € In den Shop wechseln Produktinformationen
Companion products:
Deoxynucleotide Solutions, Mix N0447S 8 µmol of each 61 € In den Shop wechseln Produktinformationen
N0447L 40 µmol of each 244 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

Legal notice
Licenses/Patents/Disclaimers:
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. Phusion® is a registered trademark and property of Thermo Fisher Scientific.
Notice to purchaser: Limited license.
The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.
The purchase price of this product includes a limited, non-transferable license under a patent owned by New England Biolabs, Inc., to use this product. No other license under this patent is conveyed expressly or by implication to the purchaser by the purchase of this product.
Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based Phusion Hot Start Flex DNA Polymerase for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based Phusion Hot Start Flex DNA Polymerase requires a license from New England Biolabs, Inc. Please contact busdev@neb.com for more information.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.