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Isothermal Amplification

Isothermal Amplification is becoming more and more popular as a simple and cost effective alternative to standard PCR.

Your general advantages:

  • No PCR Cycler necessary
  • Fast protocols
  • High reliability & specificity

The most famous “isoAmp” is LAMP.

Videos, Schematic illustration

So what is LAMP (Loop-mediated isothermal Amplification) exactly?

Did you know that this method of Isothermal Amplification can be performed in only 5-10 minutes without big technical equipment like a PCR Cycler or similar devices?

Learn about this and more in our new video-tutorial “Overview of Loop-mediated isothermal Amplification”.

Schematic illustration LAMP

Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA. A strand-displacing DNA polymerase initiates synthesis and 2 of the primers form loop structures to facilitate subsequent rounds of amplification.

LAMP scheme

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Watch video

NEB Scientists have recently published a well respected paper on highly-specific detection of pathogens of the tropical disease “river blindness” through LAMP and a simple color change.

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Watch video

Visualizing the successful Isothermal Amplification using a simple color change! How does it work?

For more information on LAMP, please visit also www.neb.com

WarmStart LAMP Kits

For simple and reliable LAMP experiments as an alternative to PCR we now offer WarmStart LAMP Kit (DNA & RNA) and WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA).

These Master Mixes contain – by NEB’s scientists in silico created, unique Designer Versions of Bst DNA Polymerase and a Reverse Transcriptase  – optimized fpr Loop-mediated Isothermal Amplification on DNA and RNA.

The new WarmStart LAMP 2X Master Mixes (DNA & RNA) are best suited as an fast and reliable analyzing method alternative for PCR in any Molecular Biology lab as well as in the field or DNA tests outside the lab.

Advantages:
  • Reliable amplification of DNA and RNA templates in only 5-50 minutes!
  • Simple, isothermal application without a PCR Cycler.
  • Detection of amplification through fluorescence (#E1700) or by eye through color change (#M1800).

Product table

 

PRODUCT Prod. # SIZE PRICE
WarmStart® LAMP Kit (DNA & RNA) E1700S 100 rxns 212 € In den Shop wechseln Produktinformationen
WarmStart® LAMP Kit (DNA & RNA) E1700L 500 rxns 848 € In den Shop wechseln Produktinformationen
WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800S 100 rxns 212 € In den Shop wechseln Produktinformationen
WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800L 500 rxns 848 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.