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Isothermal Amplification

Isothermal Amplification is becoming more and more popular as a simple and cost-efficient alternative to standard PCR.

How does LAMP (Loop-mediated isothermal amplification) work?

Loop-mediated isothermal amplification (LAMP) uses 4-6 primers to amplify a specific DNA fragment at a constant temperature. A strand-displacing DNA polymerase initiates synthesis and two specially designed primers form loop structures to facilitate subsequent rounds of amplification through extension on the loops and additional annealing of primers.

Advantages:
  • No PCR Cycler necessary
  • Fast protocols
  • High reliability & specificity

Schematic illustration LAMP, Featured Article & Videos

Did you know that this method of Isothermal Amplification can be performed in only 5-10 minutes without sophisticated technical equipment like a PCR cycler or similar devices?

Learn more about this method in our new video-tutorial “Overview of Loop-mediated isothermal Amplification”.

Watch this video

NEB Scientists have recently published a well respected paper on highly-specific detection of pathogens of the tropical disease “river blindness” through LAMP and a simple color change.

Watch this video

Visualizing the successful Isothermal Amplification using a simple color change! How does it work?

Watch this video

For more information on LAMP, please visit also www.neb.com

With NEBs convenient WarmStart LAMP Kits, your LAMP experiments will be easy and reliable:

NEB offers a range of different tools for your LAMP/RT-LAMP toolbox with ~20 min protocols that serve as a fast and reliable alternative to classical PCR in any molecular biology lab or even in field trials or DNA testing outside a lab.

As a “universal” solution, we recommend using the WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG). This Master Mix uses the WarmStart RTx and the Bst 2.0 WarmStart DNA Polymerase which are unbeatable in their performance. The Mix is compatible with many detection methods, including color change, fluorescence, turbidity measurement or agarose gel. The detection of the amplification is pH independent. Thus, this master mix can also be used for DNA or RNA samples in strongly buffered or acidic solution.

This “universal” master mix is also available in a useful kit version, the WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG), which includes a separate vial of 50x LAMP Fluorescent Dye.

LAMP Kit Detektionsmethoden

The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is compatible with different detection methods including color change, fluorescence, turbidity measurement or agarose gel.

Advantages:
  • Reliable amplification of DNA or RNA templates in only 5-50 min!
  • Easy, isothermal application, no PCR cycler required
  • Compatible with different detection methods, such as turbidity or fluorescence or by color change – for detection by eye!

Using the WarmStart Colorimetric LAMP 2x Master Mixes, DNA amplification can be detected by eye – thanks to a simple, visual pH-dependent color change.

In our colorimetric LAMP/RT-LAMP kits, the pH of the reaction changes during amplification in the course of dTNP incorporation to a slight acidic value. Thus, a successful amplification can be detected as a visible color change that is particularly easy to read out.

pH-Wert-abhängige colorimetrische LAMP Detektion

The colorimetric kits are therefore often used outside the lab, e.g. in point-of-care coronavirus diagnostics (more info), in field studies for zika virus detection within the World Moscito Program (more info) or even in space.

genesinspace_banner.jpg

Genes in Space:
Diagnostic DNA Detection without PCR – first successful LAMP-detection in outer space.

Find our more!

Product table

 

PRODUCT Prod. # SIZE PRICE
WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix with UDG M1708S 100 rxns 264 € NEB Shop_icon NEB Info_icon
WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix with UDG M1708L 500 rxns 1.057 € NEB Shop_icon NEB Info_icon
WarmStart Fluorescent LAMP/RT-LAMP Kit with UDG E1708S 100 rxns 273 € NEB Shop_icon NEB Info_icon
WarmStart Fluorescent LAMP/RT-LAMP Kit with UDG E1708L 500 rxns 1.092 € NEB Shop_icon NEB Info_icon
WarmStart Colorimetric LAMP 2X Master Mix with UDG M1804S 100 rxns 270 € NEB Shop_icon NEB Info_icon
WarmStart Colorimetric LAMP 2X Master Mix with UDG M1804L 500 rxns 1080 € NEB Shop_icon NEB Info_icon
SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit E2019S 96 rxns 698 € NEB Shop_icon NEB Info_icon
WarmStart® LAMP Kit (DNA & RNA) E1700S 100 rxns 218 € In den Shop wechseln Produktinformationen
WarmStart® LAMP Kit (DNA & RNA) E1700L 500 rxns 874 € In den Shop wechseln Produktinformationen
WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800S 100 rxns 218 € In den Shop wechseln Produktinformationen
WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800L 500 rxns 874 € In den Shop wechseln Produktinformationen

As of: 19.08.2021

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.