Isothermal Amplification is becoming more and more popular as a simple and cost effective alternative to standard PCR.
Your general advantages:
- No PCR Cycler necessary
- Fast protocols
- High reliability & specificity
The most famous “isoAmp” is LAMP.
Videos, Schematic illustration
So what is LAMP (Loop-mediated isothermal Amplification) exactly?
Did you know that this method of Isothermal Amplification can be performed in only 5-10 minutes without big technical equipment like a PCR Cycler or similar devices?
Learn about this and more in our new video-tutorial “Overview of Loop-mediated isothermal Amplification”.
Schematic illustration LAMP
Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA. A strand-displacing DNA polymerase initiates synthesis and 2 of the primers form loop structures to facilitate subsequent rounds of amplification.
NEB Scientists have recently published a well respected paper on highly-specific detection of pathogens of the tropical disease “river blindness” through LAMP and a simple color change.
Visualizing the successful Isothermal Amplification using a simple color change! How does it work?
WarmStart LAMP Kits
These Master Mixes contain – by NEB’s scientists in silico created, unique Designer Versions of Bst DNA Polymerase and a Reverse Transcriptase – optimized fpr Loop-mediated Isothermal Amplification on DNA and RNA.
The new WarmStart LAMP 2X Master Mixes (DNA & RNA) are best suited as an fast and reliable analyzing method alternative for PCR in any Molecular Biology lab as well as in the field or DNA tests outside the lab.
- Reliable amplification of DNA and RNA templates in only 5-50 minutes!
- Simple, isothermal application without a PCR Cycler.
- Detection of amplification through fluorescence (#E1700) or by eye through color change (#M1800).
As of: 01.01.2020