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OneTaq DNA Polymerase

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases for use with routine and difficult PCR experiments. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.

OneTaq DNA Polymerase is supplied with two 5X buffers (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.

Advantages:

  • Ideal for routine, AT- and GC-rich PCR Applications

Applications:

  • High Sensitivity PCR
  • High Throughput PCR
  • Routine PCR
  • GC-rich PCR
  • AT-rich PCR
  • Colony PCR
  • Long PCR (up to ~6 kb genomic)

New:
OneTaq Quick-Load DNA Polymerase – After PCR directly onto the gel!
Besides the conventional reaction buffer this new variant contains a colored Quick-Load reaction buffer for direct gel loading on agarose gels after PCR. OneTaq Quick-Load DNA Polymerase is therefore particularly well suited for PCR like genotyping, colony PCR etc. (up to 6 kb and ca. 55% GC content).
Quick – reliable – favorable!

Whichever option you choose – the OneTaq DNA Polymerase is the unmatched standard for your everyday PCR.

OneTaq DNA Polymerase

Experience the advantages and request your free OneTaq sample here!

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Application Note:

“Robust Colony PCR from Multiple E. coli Strains
using OneTaq® Quick-Load® Master Mixes”

Download PDF
NEB_ColonyPCR_ApplicationNote

Amplification of a selection of sequences with varying GC content from human and C. elegansgenomic DNA using OneTaq DNA Polymerase. GC content is indicated above gel. Marker M is the 1 kb DNA Ladder .

OneTaq Hot-Start DNA Polymerase

OneTaq® Hot Start DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases combined with an aptamer-based inhibitor.
The inhibitor binds reversibly, blocking polymerase activity at temperatures below 45°C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. OneTaq Hot Start DNA Polymerase does not require a separate high temperature incubation step to activate the enzyme and can be used in typical Taq-based cycling protocols.

Prolonged incubation times at room temperature have no influence on the performance of OneTaq® Hot Start DNA Polymerase

Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq Hot Start DNA Polymerase. The presence or absence of an extended room temperature incubation does not affect performance. GC content is indicated below gel. Marker M is the 1 kb DNA Ladder .

Comparison of recommended activation steps of commercially available Hot Start DNA Polymerases

 MANUFACTURER  ENZYME  ACTIVATION STEP*  HOT START FORM
 Applied BioSystems  AmpliTaq Gold® 360  10′, 95°C  Modified
 Invitrogen  Platinum® Taq  30″–2′, 94°C  Ab
 Promega  GoTaq® Hot Start  2′, 94–95°C  Ab
 Qiagen  HotStarTaq  15′, 95°C  Modified
 Roche  FastStart Taq  4′, 95°C  Modified
 Sigma  JumpStart™ Taq  1′, 94°C  Ab
 Thermo Fisher  Thermo-Start Taq  15′, 95°C  Modified
 NEB  OneTaq  None  Aptamer

Product table

PRODUCT Prod. # SIZE PRICE
OneTaq® DNA Polymerase M0480S 200 units 38 € In den Shop wechseln Produktinformationen
M0480L 1.000 units 152 € In den Shop wechseln Produktinformationen
M0480X 5 x 1000 units 600 € In den Shop wechseln Produktinformationen
OneTaq® Quick-Load® DNA Polymerase M0509S 100 units 20 € In den Shop wechseln Produktinformationen
M0509L 500 units 80 € In den Shop wechseln Produktinformationen
M0509X 2500 units 340 € In den Shop wechseln Produktinformationen
OneTaq® Hot Start DNA Polymerase M0481S 200 units 78 € In den Shop wechseln Produktinformationen
M0481L 1000 units 312 € In den Shop wechseln Produktinformationen
M0481X 5 x 1000 units 1.248 € In den Shop wechseln Produktinformationen
OneTaq® 2X Master Mix with Standard Buffer M0482S 100 rxns 39 € In den Shop wechseln Produktinformationen
M0482L 500 rxns 156 € In den Shop wechseln Produktinformationen
OneTaq® Hot Start 2X Master Mix with Standard Buffer M0484S 100 rxns (50 µl vol) 66 € In den Shop wechseln Produktinformationen
M0484L 500 rxns 272 € In den Shop wechseln Produktinformationen
OneTaq® Hot Start 2X Master Mix with GC Buffer M0485S 100 rxns 66 € In den Shop wechseln Produktinformationen
M0485L 500 rxns 272 € In den Shop wechseln Produktinformationen
OneTaq® Quick-Load 2X Master Mix with Standard Buffer M0486S 100 rxns 40 € In den Shop wechseln Produktinformationen
M0486L 500 rxns 160 € In den Shop wechseln Produktinformationen
M0486X 5x 500 rxns 708 € In den Shop wechseln Produktinformationen
OneTaq® Hot Start Quick-Load 2X Master Mix, Standard Buffer M0488S 100 rxns 69 € In den Shop wechseln Produktinformationen
M0488L 500 rxns 282 € In den Shop wechseln Produktinformationen
OneTaq® Hot Start Quick-Load 2X Master Mix with GC Buffer M0489S 100 rxns 69 € In den Shop wechseln Produktinformationen
M0489L 500 rxns 282 € In den Shop wechseln Produktinformationen
Companion products:
Deoxynucleotide Solutions, Mix N0447S 8 µmol of each 61 € In den Shop wechseln Produktinformationen
N0447L 40 µmol of each 244 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.