OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases for use with routine and difficult PCR experiments. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.
OneTaq DNA Polymerase is supplied with two 5X buffers (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.
- Ideal for routine, AT- and GC-rich PCR Applications
- High Sensitivity PCR
- High Throughput PCR
- Routine PCR
- GC-rich PCR
- AT-rich PCR
- Colony PCR
- Long PCR (up to ~6 kb genomic)
“Robust Colony PCR from Multiple E. coli Strains
using OneTaq® Quick-Load® Master Mixes”
OneTaq Hot-Start DNA Polymerase
OneTaq® Hot Start DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases combined with an aptamer-based inhibitor.
The inhibitor binds reversibly, blocking polymerase activity at temperatures below 45°C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. OneTaq Hot Start DNA Polymerase does not require a separate high temperature incubation step to activate the enzyme and can be used in typical Taq-based cycling protocols.
Prolonged incubation times at room temperature have no influence on the performance of OneTaq® Hot Start DNA Polymerase
Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq Hot Start DNA Polymerase. The presence or absence of an extended room temperature incubation does not affect performance. GC content is indicated below gel. Marker M is the 1 kb DNA Ladder .
Comparison of recommended activation steps of commercially available Hot Start DNA Polymerases
|MANUFACTURER||ENZYME||ACTIVATION STEP*||HOT START FORM|
|Applied BioSystems||AmpliTaq Gold® 360||10′, 95°C||Modified|
|Invitrogen||Platinum® Taq||30″–2′, 94°C||Ab|
|Promega||GoTaq® Hot Start||2′, 94–95°C||Ab|
|Roche||FastStart Taq||4′, 95°C||Modified|
|Sigma||JumpStart™ Taq||1′, 94°C||Ab|
|Thermo Fisher||Thermo-Start Taq||15′, 95°C||Modified|
As of: 01.01.2021